【摘要】 小麦成熟期穗发芽是世界性的自然灾害,严重影响小麦的产量和品质。Viviparous-1(Vp-1)是促进胚成熟和休眠的主要转录调节因子,与小麦穗发芽抗性有着密切的关系。本实验根据小麦Vp-1基因序列,以植物表达载体pAHC25为基础,成功构建了含有反向重复序列的RNA干扰表达载体pAHC-WVpRi。采用基因枪法轰击小麦品种新春9号幼胚材料1825个,共获得34株T0再生植株。利用Bar基因引物和干扰片段特异引物对再生植株进行PCR检测,获得Bar基因和干扰片段均为阳性的植株3株,转化率为0.16%。本研究为深入分析Vp-1基因功能,进而通过分子育种进行小麦穗发芽抗性的遗传改良提供了科学依据。更多还原

【Abstract】 Pre-harvest sprouting (PHS),as a worldwide natural disaster,seriously affects the quality and yield of wheat. Vp-1(Viviparous-1) gene is an important regulator which promotes embryo maturation and dormancy,and play an important role in wheat PHS resistance. In this study,according to the Vp-1 sequence from wheat,the RNAi expression vector of Vp-1 gene named pAHC-WVpRi was successfully constructed and transformed into wheat variety Xinchun 9. In total,1825 embryos were bombarded. A total of 34 regenerated plants with resistance to Bialaphos were obtained. Among them,three transgenic lines both with Bar gene and interference fragment were identified,with the transformation frequency of 0.16%. This laid a basis for the function analysis of Vp-1 gene and wheat improvement for PHS resistance through molecular breeding.更多还原