【摘要】 目的探讨不同温度热疗对宫颈癌Caski细胞坏死和凋亡的影响。方法采用水浴对宫颈癌Caski细胞行43℃,45℃,47℃实验组和37℃对照组热疗,四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)检测细胞增殖抑制率,流式细胞仪测定细胞凋亡和坏死率,免疫组化检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达。结果45℃,47℃组的细胞增殖抑制率在24h、48h、72h、96h分别为76.11%±5.32%、81.08%±4.03%、74.71%±3.78%、72.16%±4.75%和88.57%±3.77%、91.33%±2.43%、91.09%±1.17%、92.05%±1.67%,明显高于43℃组的26.57%±3.46%、25.49%±2.76%、22.80%±3.16%、26.87%±4.01%(q=10.41,18.23,14.53,13.57,P<0.05;q=14.41,21.62,16.03,16.01,P<0.05)。45℃与47℃组细胞增殖抑制率在24h、72h、96h的差异无显著性(q=3.01,1.49,2.44,P>0.05)。45℃组的细胞凋亡率最高,为12.82%±1.77%,与其他各组之间的差异具有显著性(F=18.69,P<0.05)。47℃组的细胞坏死率最高,为39.15%±5.67%,各组之间的差异均有显著性(F=69.16,P<0.05)。PCNA的表达随着温度的增加而降低,45℃,47℃组的平均光密度值(average optical density,AOD)分别是0.22±0.02、0.21±0.01,与37℃对照组0.28±0.02相比差异具有显著性(q=4.75,5.76,P<0.05)。结论45℃及以上的热疗能够明显抑制Caski细胞的增殖,增加凋亡和坏死率,降低PCNA的表达。更多还原

【Abstract】 Objective To investigate the effect of hyperthermia at different temperatures on the proliferation and apoptosis of Caski cells.Methods Caski cells were incubated at 43 ℃,45 ℃,47 ℃,or 37 ℃(control)in temperature-controlled water baths.The cell growth,apoptosis rate,and expression of PCNA protein were measured by MTT,flow cytometry,and immunohistochemistry respectively after the therapy.Results The inhibition rates of the cell growth in 45 ℃ and 47 ℃ groups at hours 24,48,72,and 96 were 76.11%±5.32%,81.08%±4.03%,74.71%±3.78%,and 72.16%±4.75%,and 88.57%±3.77%,91.33%±2.43%,91.09%±1.17%,and 92.05%±1.67%,respectively,which were significantly higher than those in the 43 ℃ group 26.57%±3.46%,25.49%±2.76%,22.80%±3.16%,and 26.87%±4.01%(q=10.41,18.23,14.53,and 13.57,P<0.05;and q=14.41,21.62,16.03,and 16.01,P<0.05;respectively).The difference in the inhibition effect of cell growth between 45 ℃ and 47 ℃ groups was not significant at 24,72,and 96 hours(q=3.01,1.49,2.44,P>0.05).The apoptosis rate in the 45 ℃ group(12.82%±1.77%)was significantly higher than the other groups(F=18.69,P<0.05);while the highest necrosis rate was found in the 47 ℃ group(39.15%±5.67%,F=69.16,P< 0.05).The expression of PCNA decreased with the temperature of the bath.The average optical density of PCNA was 0.22±0.02 and 0.21±0.01 respectively in the 45 ℃ and 47 ℃ groups,which were significantly higher than that in the control(0.28±0.02,q=4.75,5.76,P<0.05).Conclusions Hyperthermia at 45 ℃ or higher temperature can remarkably inhibit the proliferation of Caski cells,increase the rates of apoptosis and necrosis,and decrease the expression of PCNA protein.更多还原