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杨树叶中Na~+的积累与其耐盐能力的关系
Relationship between Accumulation of Na~+ in Leaves and Salt Tolerance of Different Populus Genotypes
【摘要】 以受体杨树品种‘107号’和转基因杨树‘18-1’的一年生枝条为材料,采用Hoagland营养液水培方法,检测二者植株生长以及根、茎、叶中Na+和K+含量变化的结果表明,在含100mmol·L-1NaCl的Hoagland营养液中,二者的生长速度都受到明显抑制,但后者的受抑程度较小;叶中Na+含量呈持续增加趋势,9d后其叶中的Na+含量显著高于前者。在二者叶片枯萎程度相同的情况下,‘18-1’叶中的Na+含量是‘107号’的1.6倍左右。在4mol·L-1NaCl溶液中,它们的表皮细胞死亡率分别为42%和97%。说明后者比前者有更高的耐盐能力。
【Abstract】 Plant growth and changes of Na+ and K+ concentrations in leaves,stems or roots were investigated in 1-year-old cuttings from receptor poplar ’107’(Populus×euramericana ’Neva’) and transgenic poplar ’18-1’,which were exposed to salt stress in Hoagland solution.It was shown that the growth of both poplars were markedly inhibited in Hoagland with 100 mmol·L-1 NaCl addition,while ’18-1’ was less inhibited than ’107’.The concentration of Na+ in leaves was keeping increasing,and after 9 day culture Na+ concentration in the leaves of ’18-1’ was greatly higher than that of ’107’.The accumulation amount of Na+ in ’18-1’ leaves was about 1.6 times higher than that in ’107’ when their leaves displayed same necrosis degree under NaCl stress.The death ratio of epidermal cells was about 42% and 97% in ’18-1’ and ’107’ in distilled water with 4 mol·L-1 NaCl addition,respectively.These results indicated that salt tolerance of the transgenic poplar ’18-1’ was much higher than that of receptor poplar ’107’.
- 【文献出处】 植物生理学通讯 ,Plant Physiology Communications , 编辑部邮箱 ,2009年11期
- 【分类号】S792.11
- 【被引频次】5
- 【下载频次】125