【摘要】 依据烟草质体全基因组序列设计引物,以甘薯质体基因组DNA为模板,PCR扩增包含质体accD基因完整编码区在内的一段序列(GenBank登录号为GQ395771)。序列分析表明:该片段全长为2209bp,包括1548bp的accD基因编码序列,推测编码515个氨基酸的蛋白质,该蛋白序列具有异质型β-CT中保守的锌指结构和C末端5个基元。同时绘制了该DNA片段的限制性酶切图谱。相似性比较显示,甘薯accD基因与大豆、马铃薯、拟南芥、人参、莴苣、葡萄、海岛棉、甘蓝、辣椒、菠菜、番茄和烟草的accD基因核苷酸相似性为72%～87%,氨基酸相似性为58%～83%。更多还原

【Abstract】 The fragment containing plastid accD gene(GenBank accession number:GQ395771) encoding the carboxyltransferase β subunit of acetyl-CoA carboxylase(ACCase) was cloned from plastid genome DNA in sweet potato(Ipomoea batatas).Sequencing analysis indicated that sweet potato accD is 2 209 bp with an 1 548 bp open reading frame(ORF),encoding a 515-amino-acid polypeptide which contained the zinc finger structure and the C-terminal regions with five motifs.The restriction map of this cloned fragment was also established.The BLAST results showed that the homologies of this gene with soybean,potato,tobacco,Arabidopsis thaliana,ginseng,lettuce,grape,sea-island cotton,cabbage,pepper,spinach,tomato and tobacco were from 72% to 87%,and the similarities of the amino acid sequences were from 58% to 83%.更多还原