【摘要】 目的:建立简便快速梯度淋洗离子色谱法直接测定尿液中草酸含量。方法:选用IonpacAS18阴离子分析柱,0~45 mmol/L KOH淋洗液,流速为1.0 ml/min,电导检测器。样品经高速离心用0.25μm孔径的微孔滤膜过滤稀释后直接进样。结果:本法相关性良好(r=0.9996),精密度高(RSD%<5.0),样品加标平均回收率为87.1%~102%,最低检出量0.008μg/ml。结论:该法操作简单、快速、准确、灵敏,适用于大批尿样分析。更多还原

【Abstract】 Objective:An ICS method was applied to simultaneously determine oxalate in oxalate.Methods:The method was based on ICS separation under the condition of isocratic eluent with 0～45 mmol/L KOH and flow rate of 1.0 ml/min by using of the IonPAC AS11 analytical column and suppressed conductivity detector.Results:It was showed that this method had good relativity(r>0.9996) and good analytic precision(RSD%<5.0).The samples′average recoveries rates were 87.1%～102% and the detection limit 0.008 μg/ml.Conclusion:Its advantages are simple operation,quickness,high sensitivity and precise results.This method can be applied widely and suitable for trace analysis of water samples.更多还原