【摘要】 本文对烟草赤星病菌Alternaria longipes产生的角质酶进行了纯化及性质测定。在含有苹果角质(诱导物)的液体培养基中培养A.longipes,第8 d达到产酶高峰。发酵液经硫酸铵沉淀、DEAE(diethylaminoethyl)Sepharose、Sephacryl S-100 HR和Phenyl-Sepharose 6 fast flow(high sub)进行纯化。通过SDS-PAGE测得蛋白分子量为59.5 kDa。该酶的最适温度为35℃,最适pH为9.0,在温度20℃～40℃及pH 4.0～10.0范围内,酶活较稳定。一些金属离子如Na+、Cu2+和Mn2+可以部分抑制角质酶活性。更多还原

【Abstract】 Cutinase from Alternaria longipes was purified and characterized.A.longipes was grown in a li-quid culture medium containing apple cutin as the inducer.After 8 d of growth,the cutinase was purified through ammonium sulphate precipitation,DEAE(diethylaminoethyl) Sepharose,Sephacryl S-100 HR,and Phenyl-Sepharose 6 fast flow(high sub).A single band was observed by SDS-PAGE,corresponding to a molecular weight of 59.5 kDa.Optimum temperature and pH for the cutinase activity were 35℃ and pH 9.0,res-pectively.The enzyme activity was stable within 20℃-40℃ and pH 4.0-10.0.Na+,Cu2+ and Mn2+ could partially inhibit the cutinase activity.更多还原