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一个二粒小麦LRR类受体蛋白激酶基因的克隆和鉴定
Cloning and characterization of a LRR-receptor-like protein kinase gene from Triti-cum turgidum
【Abstract】 Near the HMW-glutenin gene of wheat (Triticum aestivum), there is a locus (temporarily named TaXa) encoding LRR-receptor-like protein kinase, which is homologous to disease resistance protein Xa21 of rice (Oryza sativa) . Through RT-PCR approach, a cDNA clone of ZS2002 was isolated from the orthologous locus of TaXa in Triticum turgidum. ZS2002 was 3 081 bp long and encoding a peptide composed of 1 026 amino acid. The protein included N-terminal conserved sequence, LRR domains, a transmembrane region and a serine/threonine protein kinase domain. ZS2002 was expressed in root, stem, leaf and spike. The transcribing in seedling leaves was significantly enhanced by Blumeria graminis f.sp. tritici. TaXa gene might play a role in powdery mildew resistance reaction in Triticum.
【Key words】 Triticum turgidum; leucine-rich repeat (LRR); receptor-like protein kinase (RLK); cloning; characterization;
- 【文献出处】 植物病理学报 ,Acta Phytopathologica Sinica , 编辑部邮箱 ,2009年02期
- 【分类号】S512.1
- 【被引频次】4
- 【下载频次】208