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一个二粒小麦LRR类受体蛋白激酶基因的克隆和鉴定

Cloning and characterization of a LRR-receptor-like protein kinase gene from Triti-cum turgidum

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【作者】 牛吉山常阳刘瑞倪永静

【Author】 NIU Ji-shan, CHANG Yang, LIU Rui, NI Yong-jing (National Center of Engineering and Technological Research for Wheat, Henan Agricultural University, Zhengzhou 450002, China)

【机构】 河南农业大学国家小麦工程技术研究中心

【Abstract】 Near the HMW-glutenin gene of wheat (Triticum aestivum), there is a locus (temporarily named TaXa) encoding LRR-receptor-like protein kinase, which is homologous to disease resistance protein Xa21 of rice (Oryza sativa) . Through RT-PCR approach, a cDNA clone of ZS2002 was isolated from the orthologous locus of TaXa in Triticum turgidum. ZS2002 was 3 081 bp long and encoding a peptide composed of 1 026 amino acid. The protein included N-terminal conserved sequence, LRR domains, a transmembrane region and a serine/threonine protein kinase domain. ZS2002 was expressed in root, stem, leaf and spike. The transcribing in seedling leaves was significantly enhanced by Blumeria graminis f.sp. tritici. TaXa gene might play a role in powdery mildew resistance reaction in Triticum.

【基金】 河南省杰出人才创新基金(0621001700)
  • 【文献出处】 植物病理学报 ,Acta Phytopathologica Sinica , 编辑部邮箱 ,2009年02期
  • 【分类号】S512.1
  • 【被引频次】4
  • 【下载频次】208
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