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加拿大进境大豆上检出菜豆荚斑驳病毒

Detection of Bean pod mottle virus in soybean imported from Canada

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【作者】 沈建国王念武翁瑞泉黄可辉郭琼霞

【Author】 Shen Jianguo,Wang Nianwu,Weng Ruiquan,Huang Kehui,Guo Qiongxia(Fujian Exit-Entry Inspection and Quarantine Bureau,Fujian Key Laboratory for Technology Research of Inspection and Quarantine,Fuzhou 350001,China)

【机构】 福建出入境检验检疫局福建省检验检疫技术研究重点实验室

【摘要】 本研究采用DAS-ELISA、IC-RT-PCR及序列测定方法,对加拿大进境大豆种子进行菜豆荚斑驳病毒(Beanpod mottle virus,BPMV)和大豆花叶病毒(Soybean mosaic virus,SMV)检测,结果表明,BPMV的DAS-ELISA和IC-RT-PCR检测结果均为阳性,且PCR扩增产物序列与已报道的BPMV基因序列相似性达97%以上,而SMV的DAS-ELISA和IC-RT-PCR检测结果都为阴性。综合血清学、分子生物学检测结果,确认该批大豆携带有菜豆荚斑驳病毒。

【Abstract】 The presence of BPMV and SMV in soybean seeds imported from Canada was determined using DAS-ELISA,IC-RT-PCR and sequence analysis.The results showed that both DAS-ELISA and IC-RT-PCR were positive for BPMV,and the nucleotide sequence of BPMV PCR product had more than 97% similarity to previously reported genomic sequence.However,negative DAS-ELISA and IC-RT-PCR results were obtained for SMV.According to the results of serological and molecular biological methods,it was confirmed that BPMV was present in tested soybean imported from Canada.

【关键词】 菜豆荚斑驳病毒检测DAS-ELISAIC-RT-PCR
【Key words】 Bean pod mottle virusdetectionDAS-ELISAIC-RT-PCR
【基金】 福建省自然科学基金项目(B0610001,2006J0047);福建出入境检验检疫局科技项目(FK2007-25);福建科技重大专项专题项目(2006NZ0002-1)
  • 【分类号】S435.651;S41
  • 【被引频次】3
  • 【下载频次】67
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