节点文献
加拿大进境大豆上检出菜豆荚斑驳病毒
Detection of Bean pod mottle virus in soybean imported from Canada
【摘要】 本研究采用DAS-ELISA、IC-RT-PCR及序列测定方法,对加拿大进境大豆种子进行菜豆荚斑驳病毒(Beanpod mottle virus,BPMV)和大豆花叶病毒(Soybean mosaic virus,SMV)检测,结果表明,BPMV的DAS-ELISA和IC-RT-PCR检测结果均为阳性,且PCR扩增产物序列与已报道的BPMV基因序列相似性达97%以上,而SMV的DAS-ELISA和IC-RT-PCR检测结果都为阴性。综合血清学、分子生物学检测结果,确认该批大豆携带有菜豆荚斑驳病毒。
【Abstract】 The presence of BPMV and SMV in soybean seeds imported from Canada was determined using DAS-ELISA,IC-RT-PCR and sequence analysis.The results showed that both DAS-ELISA and IC-RT-PCR were positive for BPMV,and the nucleotide sequence of BPMV PCR product had more than 97% similarity to previously reported genomic sequence.However,negative DAS-ELISA and IC-RT-PCR results were obtained for SMV.According to the results of serological and molecular biological methods,it was confirmed that BPMV was present in tested soybean imported from Canada.
【Key words】 Bean pod mottle virus; detection; DAS-ELISA; IC-RT-PCR;
- 【文献出处】 植物保护 ,Plant Protection , 编辑部邮箱 ,2009年06期
- 【分类号】S435.651;S41
- 【被引频次】3
- 【下载频次】67