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25份北欧小麦抗白粉性评价及抗白粉病基因标记鉴定(英文)

Evaluation of 25 Nordic wheat accessions for resistance to powdery mildew and the identification of Pm genes using molecular markers

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【作者】 王俊美刘红彦康振生Buchenauer Heinrich宋玉立王瑞

【Author】 Wang Junmei1 Liu Hongyan1 Kang Zhensheng2 Buchenauer Heinrich3Song Yuli1 Wang Rui1(1.Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan Province,China;2.Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,Northwest A & F University,Yangling 712100,Shaanxi Province,China;3.Plant Protection College and Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest A & F University,Yangling 712100,Shaanxi Province,China)

【机构】 河南省农业科学院植物保护研究所西北农林科技大学陕西省农业分子生物学重点实验室西北农林科技大学植保资源与病虫害治理教育部重点开放实验室

【摘要】 采用苗期人工接菌的方法鉴定了25份北欧小麦对河南省7个不同地区小麦白粉病菌分离物的抗性,同时利用与已知抗白粉病基因Pm2、Pm3f、Pm4、Pm6、Pm13、Pm16、Pm21和Pm24连锁的分子标记对其中抗性优良的材料进行基因检测。结果表明,8份材料对所有接种的白粉病菌菌株表现高抗或免疫;其中NGB7476和NGB7809携带Pm4a和Pm16基因,NGB7481和NGB9954携带Pm6和Pm16基因,NGB9955和NGB9956同时携带Pm4a、Pm6和Pm16基因,8份材料均不携带Pm2、Pm3f、Pm4b、Pm13、Pm21和Pm24基因。

【Abstract】 Twenty-five Nordic wheat accessions was inoculated at the seedling stage with seven powdery mildew isolates from different wheat fields of Henan Province,China in order to evaluate the resistance to Blumeria graminis. And molecular markers linked to known genes Pm2,Pm3f,Pm4,Pm6,Pm13,Pm16,Pm21 and Pm24 were used to test accessions for presence of the genes. The results showed that eight accessions displayed high resistance or immunity to all isolates. Two accessions (NGB7476,NGB7809) contained both Pm4a and Pm16,two accessions (NGB7481,NGB9954) contained both Pm6 and Pm16 and two accessions (NGB9955,NGB9956) contained Pm4a,Pm6 and Pm16. The genes Pm2,Pm3f,Pm4b,Pm13,Pm21 and Pm24 were absent from any of the eight accessions.

【基金】 河南省杰出青年基金(04120001400);西北农林科技大学植保资源与病虫害治理教育部重点开放实验室资助项目;现代农业产业技术体系建设专项资金;高等学校学科创新引智计划资助项目(B07049)
  • 【文献出处】 植物保护学报 ,Acta Phytophylacica Sinica , 编辑部邮箱 ,2009年05期
  • 【分类号】S512.1
  • 【下载频次】122
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