【摘要】 为了评估甲氧虫酰肼的抗性风险,在室内进行了棉铃虫对甲氧虫酰肼的抗药性选育和现实遗传力分析。采用甲氧虫酰肼对棉铃虫敏感品系进行抗性筛选,共饲养33代,汰选29代,与敏感品系相比,获得抗性指数为32.99倍的抗性品系。采用阈性状分析方法,研究棉铃虫对甲氧虫酰肼的抗性现实遗传力,并对不同致死率下的抗性发展速率进行预测。结果表明,整个29代筛选期间,棉铃虫对甲氧虫酰肼的抗性现实遗传力(h2)为0.0830,在筛选的前、中、后期,h2分别为0.0715、0.0768和0.0947。根据室内筛选结果,假设斜率为2.0(δp=0.5),抗性现实遗传力为实际筛选估计值的一半,当杀死率为80%和90%时,棉铃虫对甲氧虫酰肼的抗性增加10倍,分别需要34.48代和27.40代。田间条件下,由于等位基因频率变化、环境变异等因素的影响,抗性增加10倍则需要更长的时间。更多还原

【Abstract】 In order to evaluate the resistance risk of Helicoverpa armigera to methoxyfenozide,H.armigera was used to select for resistance to methoxyfenozide in the laboratory. After 29 times of selection one time one generation with methoxyfenozide during 33 generations,the population developed 32.99-fold resistance to the insecticide. Realized heritability (h2) of resistance in different selection stages was evaluated based on the Tabashnik’s methods. The results suggested that h2 for the entire selection experiment was 0.0830. h2 to methoxyfenozide in the early,middle and latter selection stages were 0.0715,0.0768 and 0.0947 respectively. Assuming that slope was 2.0 (δp=0.5),and the h2 was half of indoor estimation value,it required 34.48 and 27.40 generations of H.armigera to obtain 10-fold increase of methoxyfenozide in LC50 under selection pressure at 80% and 90% mortality for each generation of selection. The number of generations required for 10-fold increase would be even more in field due to the changes in allele frequency and environmental variation or both.更多还原