【摘要】 为了明确禽大肠埃希菌的耐药情况和氨基糖苷抗性基因的流行情况,用K-B纸片扩散法检测了27株致病性大肠杆菌对19种常用抗生素的耐药谱,用PCR方法检测了耐氨基糖苷抗生素分离株的4种氨基糖苷抗性基因,包括腺苷转移酶基因aadA1和aadB、乙酰转移酶基因aacA4和磷酸转移酶基因apH(3′)-Ⅱ。结果表明,氨苄青霉素、安灭菌、青霉素G和四环素的耐药菌株率高达100%,利福平的耐药菌株率达96.3%,氯霉素、红霉素的耐药菌株为70.4%,卡那霉素、环丙沙星、氟哌酸的耐药菌株率为59.3%,链霉素的耐药菌株达到63.0%。氨苄西林/舒巴坦复方制剂、复达欣、庆大霉素和磷霉素的高敏菌株率分别为100%、77.8%、74.1%和51.9%。氨基糖苷抗性基因aadA1、aacA4和apH(3′)-Ⅱ的阳性率分别是44.4%、27.8%和55.6%,aadB未捡出。这些数据提示禽致病性大肠埃希菌存在广泛的耐药谱,氨基糖苷抗性基因aadA1和apH-(3′)-Ⅱ是主要流行基因。更多还原

【Abstract】 In order to investigate the patterns of antibiotic resistance and prevalence of aminoglycoside resistant genes in pathogenic E.coli isolated from poultry,K-B disk diffusion method was used to detect the susceptibility of 27 E.coli isolates to 19 kinds of antimicrobial agents,and 4 aminoglycoside resistant genes of aminoglycoside adenyltransferases aadA1 and aadB,aminoglycoside phosphorylases apH(3′)-Ⅱ and aminoglycoside acetyltransferases aacA4 were detected by using PCR.Results indicated that the resistance strains of 27 isolates to ampicillin,amoxicillin,penicillin G and tetracin were 100%,and that to rifampicin,chloromycin,erythromycin,Kanamycin,ciprofloxacin,norfloxacin and Streptomycin were 96.3%,70.4%,70.4%,59.26%,59.3%,59.3%,and 63.0%,respectively.The percentage of sensitive E.coli isolates to Ampicillin/sulbactan,Fortum,Gentamicin and Fosfomycin were 100%,77.8%,74.1% and 51.9%,respectively.PCR results showed that 44.4%,27.8% and 55.6% of the tested isolates were positive for aadA1,aacA4 and apH(3′)-Ⅱ,respectively.The aadB gene was not detected.These data showed that the spectrum of drug-resistance of avian pathogenic E.coli was very broad,and the aminoglycoside resistance gene of apH(3′)-Ⅱ and aadA1 were leading prevalence genes.更多还原