【摘要】 为消除M蛋白抑菌现象使其得到稳定表达,本试验采用两种策略:一是将M基因截短得到编码M蛋白膜外区的基因序列(M′),构建重组表达载体pGEX-6p-M′;二是将M基因改造,在其N端和C端同时加上GST基因,即构建pGEX-6p-M-GST重组表达载体。更多还原

【Abstract】 To obtain its stable expression,we took two strategies to eliminate the antibacterial phenomenon of membrane protein′ in this work.First,the truncated fragment of PEDV M gene which encodes the exterior of the viral envelope(M′) was subcloned into pGEX-6p-1 for the construction of pGEX-6p-M′.Second,in the same way the recombinant plasmids pGEX-6p-M-GST were constructed with GST genes linking at N-terminal and C-terminal of M gene.更多还原