【摘要】 【目的】了解2006-2007年广州地区流感的病原学分布,掌握H1N1亚型流感病毒血凝素基因(HA)的遗传和进化特征。【方法】选取2006-2007年广州市19个流感监测点的流感病毒代表株共45株,应用病原学、血清学和分子生物学方法对病毒的型别进行了鉴定;选取24株H1N1亚型流感病毒进行全长HA基因的扩增、克隆与分子进化分析。【结果】A型流感病毒H1N1和B型流感病毒交替成为广州地区2006年流感的优势流行株;2006-2007年广州地区H1N1亚型流感病毒流行株在HA蛋白的抗原区、受体结合位点和潜在糖基化位点均发生了点突变;遗传进化分析结果表明,24株H1N1亚型毒株间基因同源性的平均值为98.4%;与WHO推荐的疫苗株(New Caledonia/20/1999和Solomon Islands/3/2006)相比,同源性高达96.6%和97.8%。【结论】2006年,广州地区流感的病原体为H1N1亚型流感病毒和B型流感病毒;2006-2007年广州市H1N1亚型流感病毒HA基因发生了一定程度的变异,有必要对毒株的变异加强监测;WHO推荐的2006-2007年H1N1亚型流感病毒疫苗株适用于我国人群的免疫预防。更多还原

【Abstract】 【Objective】 To investigate the pathogen distribution of flu, and understand the genetic evolution of A / H1N1 subtype of influenza virus in Guangzhou in 2006 and 2007. 【Methods】 Forty-five representative isolates were selected from 19 influenza surveillance sentinel hospitals or labs, and the subtypes of these strains were identified by the method of serology, molecular biology, and bioinformatics. The HA gene of 24 A / H1N1 isolates was then amplified, cloned, and analyzed. 【Results】 A / H1N1 and influenza B virus became predominant strain by turn in 2006 in Guangzhou. Among the A / H1N1 isolates, some point-mutations occurred at the HA antigenic sites, receptor binding sites and potential glycosylation sites in the years of 2006 and 2007. The genetic and evolutionary analysis revealed that the homology among the 24 A / H1N1 isolates, on the average, was 98.4%. Compared with the WHO recommended vaccine strains (New Caledonia / 20 / 1999 and Solomon Islands / 3 / 2006), the homologies reached up to 96.6% and 97.8%. 【Conclusion】 A / H1N1 and influenza B virus are the predominant strains in Guangzhou in 2006. The research showed that point-mutations occurred to some extent during the years of 2006 and 2007. Hence, monitor of the variant of influenza virus should be strengthened. And the WHO recommended vaccine strains of 2006 and 2007 have the immune effect on the population of our country.更多还原