【摘要】 【目的】为了研究X染色体短串联重复序列(X-STR)基因座的遗传多态性,我们检测了DXS6803、DXS981、DXS6809、DXS6789和DXS71325个基因座在广东汉族人群中的多态性。【方法】利用荧光标记引物PCR技术复合扩增上述5个基因座,并用ABIPRISM3100毛细管电泳及GeneMapperID3.1软件进行基因分型。【结果】在363个广东汉族无关个体(男性:181个,女性:182个)中5个基因座共检出54个等位基因。多态性信息含量为0.6935～0.8177;女性个体识别率为0.8976～0.9562。三联体非父排除率为0.7805～0.8467。【结论】这5个基因座多态性较高,在个体识别和亲权鉴定中具有重要的应用价值。更多还原

【Abstract】 【Objective】 To learn about the genetic diversity,we studied the five X-chromosomal STR(X-STR) loci in Guangdong Han Nationality Groups.【Methods】 The five Loci(DXS6803,DXS981,DXS6809,DXS6789,and DXS7132) were amplified in a pentaplex PCR reaction.PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer,with GeneMapper ID 3.1 Analysis Software.【Results】 A total of 363 individuals(181 unrelated male and 182 unrelated female) from Guangdong Han population were tested,54 alleles were observed for these loci.Polymorphism information content is 0.6935 ～ 0.8177.Power of discrimination in females was 0.8976 ～ 0.9562.Mean exclusion chance for X-STR in standard trios with daughters was 0.7805 ～ 0.8467.【Conclusion】 The five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing,particularly for difficult paternity deficiency cases.更多还原