【摘要】 目的:建立糖尿病性脑梗死大鼠模型,观察对比高级糖基化终产物受体(RAGE)在模型大鼠中的表达及活络育阴方对其的影响。方法:选SD大鼠以高脂高糖饮食,腹腔注射链脲佐菌素(STZ)诱导建立糖尿病模型,在此基础上再用线栓法制作大脑中动脉栓塞(MCAO)模型;利用形态学等方法,观察不同剂量和不同时间活络育阴方对糖尿病性脑梗死的脑保护作用;用ABC免疫组化方法观察活络育阴方对糖尿病性脑梗死模型大鼠脑损伤后RAGE表达的影响。结果:活络育阴方治疗糖尿病性脑梗死大鼠,在不同剂量和不同时间内大鼠脑组织RAGE表达均明显降低。结论:活络育阴方可能通过下调RAGE表达,对糖尿病性脑梗死大鼠的神经损伤起到保护作用。更多还原

【Abstract】 Objective:To set up a rat model of diabetic cerebral embolism,in order to observe and compare the expression of advanced glycosylation end product receptor(RAGE) in rat brain and effects about Huoluoyuyinfang Decoction on it.Methods: SD rats were fed on a diet enriched in fat and fructose and the rats were injected intrapertoneally with a low-dose streptozocin(STZ) to induce hyperglycemia.Then set up middle cerebral artery occlusion(MCAO) by ligation.Morphology and other methods were applied to observe the protective effect of Huoluoyuyinfang at different doses and differrnt times.Immunohistochemical ABC method was employed to observe the effect of Huoluoyuyinfang on RAGE in rat brain of cerebral infarction due to diabetes.Results: For different dosages and stages,the RAGE expression was significantly decreased in the brain tissue by Huoluoyuyinfang.Clusion: Huoluoyuyinfang Decoction may decrease the expression of RAGE and play an important protecting role against diabetic neurological impairment.更多还原