【摘要】 通过mRNA差异显示发现一个鳗弧菌刺激后在牙鲆肝脏中表达量显著增加的片段,结合RACE技术得到了该差异片段1 856 bp的全长cDNA,包含一个1 479 bp的开放阅读框,编码的蛋白质含有fibrinogenβ的C末端签名序列。同源性分析表明其氨基酸序列与鱼类以及哺乳类的fibrinogenβ都具有高度的相似性,因此可断定此基因为牙鲆的fibrinogenβ基因(GenBank登录号为EF581895)。RT-PCR分析表明,在注射鳗弧菌后,该基因在肝脏、肾脏、脾脏、腮、肠、心脏中的表达量呈现逐渐增加的趋势。推测其可能在鳗弧菌侵染中,起到了促进伤口愈合的作用,或通过和其他细胞因子结合在防御细菌的侵染中发挥免疫调节作用。更多还原

【Abstract】 A number of novel genes are identified,which are expressed in Japanese flounder(Paralichthys olivaceus) injected with Vibrio anguillarum by differential display.This paper reports the characterization of one of these novel genes,fibrinogen β.The full length cDNA is 1 856 bases long and contains an ORF of 1479 bases.Sequence analysis revealed that there was a C-terminal domain signature in the deduced amino acids.Alignment analysis showed that the amino acid sequence had a high identity with that from fishes and mammals.When challenged with Vibrio anguillarum,the expression level of flounder fibrinogen β in liver,kidney,spleen,gill,intestine and heart tissues increased obviously.These results might indicate that the flounder fibrinogen β may help stop bleeding by helping blood clots formation,or associate with other cytokines and play a critical role in the host immune response.更多还原