【摘要】 细菌性软腐病是石斛兰及其它兰花栽培和生产中的一大危害。苏云金芽孢杆菌Bacillus thuringiensis(Bt)中含有aiiA基因,该基因转入一些植物可赋予植物表现出软腐病抗性。从Bt菌中克隆出aiiA基因,并装载入植物表达载体pCAMBIA1301,构建出可用于转入石斛兰的pSAN载体;首次利用根癌农杆菌EHA105将pSAN载体转入石斛兰,并获得潮霉素抗性苗。这些抗性苗经GUS组织化学染色和PCR检测证实为转基因苗,这将为获得具有软腐病抗性的石斛兰品种打下基础。更多还原

【Abstract】 Soft rot disease is a great threat to the cultivation and production of Dendrobium and other orchids.Bacillus thuringiensis(Bt) contains aiiA gene encoding AiiA protein which could enable plants resistance to the soft rot disease.aiiA gene was amplified from Bt and constructed into the binary vector pCAMBIA1301 to construct the expression vector pSAN.For the first time,the aiiA gene was transferred into Dendrobium by Agrobacterium tumefacien EHA105.Through 4-month selection cultivation,the hygromycin-resistant shoots regenerated from PLBs.Subjected to GUS assay and PCR analysis,the shoots were proved to be transformed shoots.This research work may contribute to cultivate novel Dendrobium species which could resistant to soft rot disease.更多还原