【摘要】 目的探讨日本血吸虫生殖产卵相关基因DNA在小鼠体内组织分布以及整合到宿主基因组上的可能性。方法将生殖产卵相关基因重组质粒pcDNA3/Sj SDISP经肌注免疫小鼠后,在注射后12h、1w、3w和6w取小鼠各组织,抽提其基因组DNA进行PCR扩增,然后纯化基因组DNA进行PCR扩增,结果经琼脂糖凝胶电泳分析。结果注射后12h,在血液、心、肝、脾、肺、肾及注射部位肌肉中可检测到pcDNA3/Sj SDISP,至第1w,仍可在部分小鼠中检测到,至第3w不再检出。未发现生殖产卵相关基因pcDNA3/Sj SDISP整合入小鼠基因组中。结论肌注pcDNA3/Sj SDISP后,可在组织中广泛分布,但并未持续存在体内。未发现与宿主细胞基因组整合的直接证据。更多还原

【Abstract】 To investigate the tissue distribution of the fecundity-associated genes and the possibility of integration of these genes to the host genome in mice immunized with DNA vaccine pcDNA3/SjSDISP,this recombinant plasmid was injected to mice by intra-muscular route and various tissues were removed from the immunized mice at 12 hours,1,3 and 6 weeks after immunization.The total tissue DNAs were extracted and amplified by PCR.Then,the genomic DNA of host cells was purified from the non-integrated free-plasmid for PCR amplification and the results were identified by agarose gel electrophoresis.It was found that the plasmid DNA pcDNA3/SjSDISP could be detected in blood,heart,liver,spleen and the injected muscle loci 12 hours after immunization.It could be detected in some mice one week later but disappeared completely 3 weeks post-inoculation.In addition,there was no sign of integration of the recombinant plasmid into mouse genome.It is evident that the recombinant plasmid pcDNA3/SiSDISP distributes in a broad range of tissues after intramuscular injection,but it exists only for a limited period of time in vivo.There is no direct evidence of danger that the recombinant plasmid can integrate to the host genome.更多还原