【摘要】 目的研究维生素D受体(VDR)基因多态性与新疆维吾尔族人群结核病易感性的相关性。方法采用病例对照研究,选取224例新疆维吾尔族结核患者和225例有分枝杆菌感染史的同民族健康者分别作为病例组和对照组。用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析方法分别检测维生素D受体基因的多态性,内切酶分别为FokI和TaqI,并进行基因分型。统计学分析结果采用χ2检验,P值为0.05。结果FokI分析,分为FF、Ff、ff三种基因型,其在病例组和对照组中分别为36%、57%、7%和40%、54%、6%。FF基因型的分布在病例组与对照组中的差异性无统计学意义(χ2=0.872,P>0.05)。TaqI分析,分为TT、Tt、tt三种基因型,其在病例组和对照组中分别为68.8%、29.4%、1.8%和75.6%、22.2%、2.2%。TT基因型在病例组与对照组中的差异性也无统计学意义(χ2=2.588,P>0.05)。结论维生素D受体基因中FokI与TaqI多态性与新疆维吾尔族人群结核病易感性无明显相关性。更多还原

【Abstract】 To investigate the association of the polymorphism for the vitamin D receptor gene and the susceptibility to pulmonary tuberculosis in this population,the Fok1 and Taq1 genes for the vitamin D receptor(VDR) were typed in 224 patients with active pulmonary tuberculosis(TB) in Chinese Ughurs and 225 healthy individuals of the same population who had been contacted with TB.and the relationship of these kinds of polymorphism with the incidence of TB was studied by means of the statistical method,such as χ2 test.It was found that the percentages of the 3 genotypes of FF,Ff and ff in patients with active pulmonary TB were 36.2%(81/224),56.7%(127/224) and 7.1%(16/224) respectively;while those in the healthy controls were 40.4%(91/225),53.3%(120/225) and 6,2%(14/225) respectively.No significant difference in the distribution of the FF genotype of VDR could be detected between patients and healthy controls(χ2=0.872,P>0.05).The FF,Ff and ff genotypes in patients were 68.6%(154/224),29.4%(66/224) and 1.8%(4/224);while those in healthy controls were 75.6%(170/225),22.2%(50/225) and 2.2%(5/225) respectively,also no significance between these two groups(χ2=2.588,P>0.05).It is evident that no significant association exists in the polymorphism of the Fok1 and Taq1 loci of the vitamin D receptor gene and the susceptibility to pulmonary tuberculosis in population of Chinese Uighurs.更多还原