【摘要】 目的初步观察蓝氏贾第鞭毛虫细胞分裂过程中典型凝集染色体的形成及其数目。方法用改良TYI-S-33培养基培养贾第虫滋养体。在结束培养前4h弃去旧培养基,加入事先预热的含有秋水仙素(终浓度为0.2μg/ml)的新鲜培养液。低渗处理55min,用甲醇∶冰醋酸(体积比=3∶1)固定液固定。收集细胞、滴片、Giemsa染液染色,光学显微镜观察结果。结果获得了较好的染色体分裂相。贾第虫染色体的数目多数为2n=8条。50个细胞分裂相中,有35个为8条染色体,10个为9条染色体,5个为10条染色体。染色体微小呈小杆状,有时成对,形态相同。结论蓝氏贾第鞭毛虫在分裂过程中形成典型的凝集染色体,染色体数目为2n=10条。更多还原

【Abstract】 To observe the formation of the typical chromosomes of Giardia lamblia and their number during mitosis.The trophozoites were cultivated at 37 ℃ in modified TYI-S-33 medium,and the cell cultures were enriched with fresh,warmed medium containing colchicines(final concentration 0.2 g/mL).when the old cultural medium was removed 4 hours before the end of cultivation.After low osmosis treatment for 55 minutes,the cell suspension was fixed with a mixture solution of methanol and glacial acid(volume ratio:3∶1),stained with Giemsa stain and examined under light microscopy.In this way,a better karyotype of G.lamblia could be obtained.Mostly,the number of chromosomes was eight(2n=8).In 50 cells with mitosis,35,10 and 5 cells had 8,9 and 10 chromosones respectively.The chrompsomes appeared as a short rod-shape with tiny size and sometimes they existed in pairs with the same morphology.更多还原