【摘要】 目的分析和预测亚洲牛带绦虫泛素缀合酶基因及其编码蛋白的结构与功能特性,用于指导其生物学功能的实验研究。方法利用美国国家生物技术信息中心和瑞士生物信息学研究所的蛋白分析专家系统中有关基因和蛋白的序列和结构信息分析的各种工具,结合其它生物信息学分析软件包,如VectorNTIsuite,从亚洲牛带绦虫成虫cDNA质粒文库中识别出泛素缀合酶基因及其编码区,分析、预测该基因编码的蛋白质的理化特性、翻译后的修饰位点、功能域、亚细胞定位、拓扑结构、二级结构等。结果该序列为全长基因,编码区为76bp-537bp,编码154个氨基酸。该序列为泛素缀合酶基因同源序列。其编码蛋白的理论分子量为17397.1,亚细胞定位在细胞核。预测该蛋白无跨膜区。与吸虫属的泛素缀合酶进化关系最近。结论应用生物信息方法从亚洲牛带绦虫成虫cDNA文库中筛选出了亚洲牛带绦虫泛素缀合酶cDNA全长序列并预测得到其结构与功能方面的信息。更多还原

【Abstract】 The structure and characteristics of ubiquitin-conjugated enzyme from Tainia asiatica(T.a E2)were analyzed and predicted by bioinformatics software package and bioinformatics tools provided by websites of NCBI and ExPaSY.A full-length cDNA sequence of ubiquitin-conjugated enzyme from cDNA plasmid library of T.asiatica was identified and the characteristics of the deduced proteins including their physico-chemical properties,post-translational modification sites and domains,sub-cellular location,topological and secondary structures were predicted.It was found that the full-length cDNA sequence of ubiquitin-conjugated enzyme of T.asiatica had a complete open reading frame(ORF)of 554 bp that encoded a putative protein with 154 amino acid residues.It’s coding region situated in an area from 76 bp to 537 bp.The amino acid sequence showed high identity with the ubiquitin-conjugated enzyme of other species submitted in GenBank,while,the molecular weight of T.a E2 was predicted to be 17397.1 Da.This protein had no transmembranous region and their sub-cellular location was situated at cell nucleus.A close relationship in phylogenesis between T.a E2 and the ubiquitin-conjugated enzymes of trematode species could be demonstrated.更多还原