【摘要】 目的探索广州管圆线虫病IgM早期诊断时机。方法从感染福寿螺中分离第Ⅲ期幼虫,感染6w龄Balb/c小白鼠,分别于感染前及感染后的第1、2、3、4、8、16、21、28、38d取血。经方阵滴定确定最佳抗原包被量和血清最佳包被浓度,进一步测定感染广州管圆线虫小鼠的不同时期血清中IgM含量,同时设立阴性、阳性对照。结果小鼠血清中IgM抗体在感染后就开始升高,感染后第8dIgM抗体检测阳性率已达100%。结论感染后1w,小鼠血清中IgM检测已具有临床诊断价值。更多还原

【Abstract】 To understand the dynamic changes of IgM antibodies produced in mice experimentally infected with Angiostrongylus canonensis in order to evaluate the proper time for early diagnosis of angiostrongyliasis,BALB/c mice was infected with larvae of A.canonensis at III stage and the blood samples of the infected mice were taken before and after different times of infection to investigate the dynamic change of IgM antibodies by ELISA using the crude saline extract of larvae at IV-V stage as the coating antigen. It was found that the levels of the specific IgM antibodies against A.canonensis in sera of the infected mice gradually increased along with the progress of time after infection and reached its first peak 8 days after infection with a 100% of detection rate. This result suggests that detection of the IgM antibodies at the first week after infection exhibits significant value for the clinical diagnosis of angiostrongyliasis.更多还原