【摘要】 目的:探讨姜黄素(curcumin)对人脑胶质瘤U-251细胞ATP酶的影响及诱导胶质瘤细胞凋亡的机制。方法:分别用20、40、80和100μmol/L姜黄素处理U-251细胞24h,用MTT法测定姜黄素对U-251细胞增殖的抑制作用;流式细胞仪检测细胞凋亡,比色法测定ATP酶活力。结果:姜黄素抑制U251细胞的增殖;诱导U251细胞凋亡;姜黄素明显使细胞质膜ATP酶活力明显降低。结论:姜黄素通过抑制细胞质膜ATP酶活性可诱导胶质瘤细胞凋亡和抑制增殖。更多还原

【Abstract】 Objective:To study the effects of curcumin on the activity of ATPase and the mechanism of apoptosis in U-251 cell.Methods:U-251 cells were treated with 20,40,80,100μmol/L curcumin for 24 h and the growth inhibition rates of U-251 cells were measured with MTT method.Cell apoptosis was inspected with flow cytometry(FCM).The activities of ATPase were determined by colorimetry method.Results:Curcumin inhibited the proliferation of U-251 cells and induced apoptosis of U-251 cells.level of ATPase in U-251 plasma Membrane was low remarkably.Conclusion:Curcumin induced apoptosis and inhibited proliferation of U-251 cell via inhibition of activation of plasma Membrane ATPase.更多还原