【摘要】 溶剂的组成影响电喷雾离子(ESI)化效率。该研究观察了ESI溶剂体系中不同浓度的乙腈(ACN)对MRFA、Reserpine、ΜLtramark1621、马肌球蛋白酶切肽段等标准物质的质谱信号响应强度的影响,发现在溶剂体系中,ACN浓度达到70%时,上述物质的质谱信号强度具有最大值。根据这一现象,对传统的反相色谱-电喷雾-质谱(RPLC-ESI-MS)系统进行管路改造,在分析柱后通过1个三通阀引入适当流速的有机溶剂进行辅助喷雾。对马心肌蛋白酶切肽段混合物的分析表明,该方法可以有效提高酶切肽段的检出率,并提高鉴定蛋白质的序列覆盖率。更多还原

【Abstract】 The efficiency of electrospray ionization was affected by the component of solvent.The effect of different concentration of ACN on mass spectrometry for MRFA,Reserpine,ΜLtramark1621 and peptide mixture were observed,and found that all of the chemicals have the max mass spectra signal intensity in 70% ACN.Then,the peptide mixtures from myoglobin were analyzed using CapLC-ESI-LTQ system,which were equipped with auxiliary electrospray by 100%ACN,0.1%FA.This results show that the detection ratio of peptides and the recovery of protein sequence are remarkable improved.更多还原