【摘要】 采用电喷雾离子阱(ESI-MS)质谱技术对水飞蓟宾的结构和裂解途径进行研究。采用ESI-MS获得了m/z481[M-H]-,采用ESI-MS2获得了m/z453、m/z301等碎片离子。发现ESI-MS2质谱生成A环和B环C—O—C键断裂的碎片,并对m/z453、m/z301特征碎片离子进行ESI-MS3质谱研究,归属了其主要特征碎片离子,分析和讨论了该化合物的结构和质谱特征。更多还原

【Abstract】 The structure and fragmentation pathway of silybin were elucidated by electron spray ionization mass spectrometry(ESI-MS).Quasi-molecular ion peak m/z 481 -was detected by ESI-MS,and the ions of m/z 453 and m/z 301 were detected by ESI-MS2.The fragment routes of characteristic were discussed on the basis of ESI mass spectra.The full scan MS2 spectra of silybin gave characteristic fragment ions formed through cleavage of the C—O—C bond at A-ring and B-ring.The full scan MS3 spectra of fragment m/z 453,m/z 301 was obtained by ion trap mass spectrometry.The characteristic fragmentation was used to prove the structure of m/z 453,m/z 301.更多还原