【摘要】 目的探讨内皮抑素及碱性成纤维细胞生长因子(bFGF)在胆囊癌发生、发展中的作用,为胆囊癌的生物学治疗探索理论依据。方法运用免疫组化SP法检测内皮抑素、bFGF及CD34在61例胆囊癌组织和10例正常胆囊组织中的表达,并通过CD34的表达计算微血管密度(MVD),分析它们与胆囊癌临床病理特征的关系。结果内皮抑素在正常胆囊组织及胆囊癌组织中的表达阳性率分别为40.00%(4/10)及77.05%(47/61),差异有统计学意义(P<0.05);胆囊癌组织中,内皮抑素的表达强度与其临床分期及淋巴结转移有关(P<0.05),与患者性别及年龄?肿瘤部位和大小及组织学分级无关(P>0.05)。bFGF在正常胆囊组织和胆囊癌组织中的表达阳性率分别为20.00%(2/10)及67.21%(41/61),差异有统计学意义(P<0.05);胆囊癌组织中,bFGF的表达强度与其临床分期及淋巴结转移有关(P<0.05),与患者性别及年龄?肿瘤部位和大小及组织学分级无关(P>0.05)。胆囊癌组织中MVD为(76.66±20.15)个/HP,明显高于正常胆囊组织的(29.53±5.03)个/HP(P<0.01)。胆囊癌组织中,临床分期Ⅲ～Ⅴ期的MVD为(80.53±17.98)个/HP,明显高于Ⅰ+Ⅱ期的(46.79±5.38)个/HP(P<0.01);有淋巴结转移者的MVD为(94.60±7.28)个/HP,明显高于无淋巴结转移者的(58.12±9.24)个/HP(P<0.01);MVD在组织学分级G1(60.59±14.71)个/HP、G2(83.08±15.30)个/HP及G3(96.53±6.92)个/HP者间的差异均有统计学意义(P<0.01);MVD与患者性别及年龄?肿瘤部位及大小无关(P>0.05)。胆囊癌组织中内皮抑素的表达与MVD有关(P<0.01),bFGF的表达亦与MVD有关(P<0.01)。结论内皮抑素、bFGF及CD34在胆囊癌的发生、发展过程中起不同程度的作用,它们的检测可给胆囊癌的早期诊断、恶性程度的判定和进一步治疗提供有效的依据。更多还原

【Abstract】 Objective To study the effects of the expressions of endostatin, basic fibroblast growth factor (bFGF) and CD34 on oncogenesis and progression of gallbladder cancer, and to explore some valuable criterias for its biotherapy. Methods The expressions of endostatin, bFGF and CD34 were studied by means of immunohistochemistry (SP) in 61 cases of gallbladder cancer and 10 cases of normal cholecystic tissue, and microvessel density (MVD) was calculated by the expression of CD34. Their relationships with clinical pathological features were also investigated. Results The expression rates of endostatin in normal cholecystic tissue and in gallbladder cancer tissue were 40.00% (4/10) and 77.05% (47/61) respectively, which had statistical difference (P<0.05). The expression of endostatin in 61 cases of caner was relational to clinical stage and metastasis of lymph nodes (P<0.05), while no significant correlation was detected with sex and age of patient, location of tumor, size of tumor and histologic grade (P>0.05). The expression rates of bFGF in normal cholecystic tissue and in gallbladder cancer tissue were 20.00%(2/10) and 67.21% (41/61) respectively, which had statistical difference (P<0.05). The expression of bFGF in 61 cases of caner was relational to clinical stage and metastasis of lymph nodes (P<0.05), while no significant correlation was detected with sex and age of patient, location of tumor, size of tumor and histologic grade (P>0.05). MVD in gallbladder cancer tissue and in normal cholecystic tissue was (76.66±20.15) piece/HP and (29.53±5.03) piece/HP respectively, showing significant difference (P<0.01). In 61 cases of cancer, MVD in clinical stage Ⅲ～Ⅴ (80.53±17.98) piece/HP was much higher than that in stage Ⅰ+Ⅱ (46.79±5.38) piece/HP, P<0.01; MVD was higher in those with lymph nodes metastasis (94.60±7.28) piece/HP than those without metastasis (58.12±9.24) piece/HP, P<0.01; and MVD was (60.59±14.71) piece/HP in histologic grade G1, (83.08±15.30) piece/HP in G2, and (96.53±6.92) piece/HP in G3, the difference was significant among them (P<0.01). There was no significant correlation between MVD and sex and age of patient, location of tumor and size of tumor (P>0.05). There were statistically significant correlations between expressions of endostatin and MVD (P<0.01), expressions of bFGF and MVD (P<0.01). Conclusions The result suggests that endostatin, bFGF and CD34 play roles in oncogenesis and progression of gallbladder cancer. Detection of these proteins has positive effects on diagnosis, malignant degree determination and treatment of gallbladder cancer.更多还原