【摘要】 目的:研究三色荧光抗体染色方法测定人外周血T淋巴细胞亚群并探讨其临床应用。方法:三色荧光抗体染色流式细胞术测定15例健康志愿者及15例尖锐湿疣(CA)患者外周血Th1/Th2亚群及Tc1/Tc2亚群比例。结果:标本经流式细胞仪检测结果稳定。与健康对照组比较,CA患者Th1及Tc1细胞含量、Tc1/Tc2比值均减少(P<0.05),该结果与文献报道的采用其他方法检测结果相一致。结论:三色荧光抗体染色流式细胞术能敏感精确地测定机体外周血T淋巴细胞亚群变化情况。更多还原

【Abstract】 Objective:To investigate the detection and clinical application of T lymphocyte subsets by three-colour immunofluorescence.Methods:The proportion of Th1/Th2 and Tc1/Tc2 cells in the peripheral blood of 15 CA patients and 15 health controls were detected by three-colour immunofluorescent flow cytometry.Results:Results tested by flow cytometry are stable.Compared to health controls,Thl,Tc1 cells and Tcl/Tc2 ratio in CA patients are all decreased(P<0.05).Conclusion:Three-colour immunofluorescence flow cytometry is a precise and sensitive method in detecting T lymphocyte subsets.更多还原