【摘要】 【目的】尝试利用分子标记和QTL定位技术直接定位影响重组频率的QTL,探索物种遗传变异难易的分子基础。【方法】分别借助3张玉米和3张水稻分子标记连锁图,以所有染色体上标记的交换次数为性状进行分析。【结果】分别定位了7个和11个影响玉米和水稻重组频率的QTL。以玉米和水稻高密度分子标记连锁图IBM302和Genetic98每条染色体上标记的交换次数为性状,分别在玉米和水稻上定位了12个和57个QTL。【结论】影响重组频率的基因真实存在。培育高重组频率材料将有助于加速基因的定位和克隆、比较图位克隆基因和遗传育种进程。更多还原

【Abstract】 【Objective】Recombination plays an important role in species evolution as well as plant and animal breeding. Here, the number of crossover breakpoints was used as a trait to map the QTL controlling recombination of genomes. 【Method】 Total number of crossover breakpoints across all chromosomes (NCAC) was used as the trait. 【Result】 Seven and eleven QTL were detected in 3 maize and 3 rice genetic populations, respectively. Meanwhile, 12 and 57 more QTL were detected in the maize IBM302 and rice Genetic98 population, respectively, when using the number of crossover breakpoints per chromosome (NCPC) as the trait. The number of QTL detected is strongly correlated with the resolution of the genetic linkage map. 【Conclusion】 These results not only have provided evidences for the true existence of genes controlling recombination frequency, but also facilitate our further research on map-based gene cloning and molecular breeding.更多还原