【摘要】 利用改良的CTAB方法从香蕉叶片中提取高质量的DNA。在参考一般RAPD分析反应程序的基础上,经过反复试验,确定适合香蕉PCR扩增程序为:94℃预变性5min;94℃变性1min;38℃退火1min;72℃延伸2min;循环45次;最后72℃延伸2min。PCR扩增的体系(总体积25μl)为:模板DNA20ng,dNTP200μmol/L,10×PCRBuffer2.5μl,引物0.20μmol/L,Taq酶0.75U,ddH2O17.85μl。更多还原

【Abstract】 Using the improved method of CTAB,high purity DNA was obtained from banana leaves.Based on the common RAPD reaction program and adjusting experiments,the optimal amplification program was described as follows:94 ℃ for 5 min;45 cycles at 94 ℃ for 1 min,38 ℃ for 1 min and 72 ℃ for 2 min;72 ℃ for 2 min at last. PCR system(25 μl total volumes)contains:20 ng of genomic DNA,200 μmol/L of dNTP,2.5 μl of 10×PCR Buffer,0.20 μmol/L of primer,0.75 U Taq DNA polymerase and 17.85 μl of ddH2O.更多还原