【摘要】 探索保存在4℃2.5%重铬酸钾(K2Cr2O7)中1～30个月的微小隐孢子虫卵囊(CPO)的活性和对免疫抑制BALB/c小鼠的传染性。通过体外脱囊技术评价卵囊的活性,以BALB/c小鼠为感染模型,通过检测CPO感染小鼠的潜伏期、排卵囊数量和回肠末端绒毛中的隐孢子虫数量来评价卵囊的传染性。结果表明:保存在K2Cr2O7中1～16个月的卵囊出现脱囊;免疫抑制BALB/c小鼠在感染保存1～16个月的卵囊后3～8天开始排出大量的卵囊,且在末端回肠绒毛中寄生有大量的隐孢子虫;卵囊的保存时间与潜伏期之间存在强烈的相关性(R2=0.92)。因此,CPO在4℃K2Cr2O7中能保持活性和传染性至少16个月,K2Cr2O7是保存CPO的良好介质。更多还原

【Abstract】 The present study was undertaken to compare the viability and infectivity of Cryptosporidium parvum oocysts(CPO) that had been stored for 1,4,7,10,13,16,20,25 and 30 months at 4℃in 2.5% potassium dichromate(K2Cr2O7) ,respectively.An excystation protocol was performed in vitro to evaluate the viability of oocysts.One hundred and eighty female BABL/c mice were used to evaluate the infectivity of oocysts by detecting the prepatent period of C.parvum infection,the quantity of oocysts excreted,and the number of parasites that colonized the villi of the ileum.Results showed that CPO preserved in K2Cr2O7 for 1 to 16 months were capable of excystation in vitro and infection of mice in vivo.The prepatent period was extended from 3 to 8 days as the storage time of oocysts increased.There was a strong correlation between prepatent period and duration of oocyst storage(R2=0.92) .Large number of oocysts were isolated and purified from feces of mice inoculated oocysts preserved for 1 to 16 months in K2Cr2O7,respectively.Cryptosporidia parasitized in the terminal ilea were found in mice inoculated with oocysts stored in K2Cr2O7 for 1 to 16 months.Conclusion:C.parvum oocysts may be stored at 4℃in K2Cr2O7 for at least 16 months for the purposes of laboratoryr esearch,and K2Cr2O7 is a good medium of keeping viability and infectivity of CPO for a long time.更多还原