【摘要】 以菜籽蛋白为原料,用碱性蛋白酶对菜籽蛋白进行部分水解。结果表明,水解得到的多肽对Fenton体系产生的羟自由基有清除能力。通过正交试验,得出最优水解条件为碱性蛋白酶[E]=4000U/g,温度为50℃,pH10.0,控制水解3h,水解度14.98%的条件时,菜籽多肽的羟自由基清除能力最强,为14.65%。更多还原

【Abstract】 The hydrolyztic process of the rapeseed protein by alcalase was studied.The results indicated that the rapeseed polypeptides had activity to eliminate hydroxyl radicals in Fenton reaction.According to orthogonal test,the highest activity of rapeseed polypeptides on hydroxyl radical elimination was achieved as 14.65% when the hydrolysis was conducted under follow conditions:[E]=4000U/g,temperrature=50℃,pH 10.0,hydrolyztic time 3h,and hydrolyzing degree(DH)14.98%.更多还原