【摘要】 利用大豆粉状磷脂,采用薄膜法结合冻融法制备阿奇霉素阳离子脂质体,以高效液相色谱法为分析手段,采用反透析法测定阿奇霉素脂质体的包封率。研究了阿奇霉素脂质体配方中不同成分的比例。阿奇霉素脂质体的最佳制备条件为:水浴温度为40℃,m(阿奇霉素)∶m(磷脂)=1∶25,m(阿奇霉素)∶m(维生素E)=40∶1,m(阿奇霉素)∶m(十八胺)=2.5∶1,加入10%的麦芽糖水溶液为20mL,在此条件下,阿奇霉素阳离子脂质体的包封率为76.07%。从形态、粒径、体外释药特性等方而对阿奇霉素脂质体进行了质量评价,并研究在小鼠体内的分布。更多还原

【Abstract】 The azithromycin cationic liposome was prepared with granule Soya lecithin by thin film method combined with freeze-thawing method. The entrapment efficiency of azithromycin liposome was determined by reverse dialysis method and HPLC analysis. The influence of component pro- portion in azithromycin liposome on the entrapment efficiency was studied. The optimum conditions for azithromycin liposome preparation were: water bath temperature was 40℃, m(azithromycin)∶m(lecithin)=1∶25, m(azithromycin):m(vitamin E)=40∶1, m(azithromycin)∶m(octadecy- lamine)=2.5∶1, and 20 ml 10% maltose aqueous solution was added. In this case, the entrapment efficiency of azithromycin liposome was 76.07%. The quality of azithromycin liposome was estimated according to morphological characteristic, particle size, release profile. The tissue distribution of azithromycin liposome was studied in mice.更多还原