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益生菌产阮假丝酵母菌MYS-J1的液体通风发酵研究

Aerobic submerged fermentation of probiotic Candida utilis MYS-J1

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【作者】 张红艳岳淑宁李忠玲张强马齐

【Author】 ZHANG Hongyan1,YUE Shuning2,LI Zhongling3,ZHANG Qiang1,MA Qi2(1.Insitute of Enzyme Engineering,Shanxi Academy of Sciences,Lintong 710600,China;2.Shanxi Quality Supervision and Inspection Station of Brewed & Fermented Product,Lintong 710600,China;3.Enzyme Engineering Technology Center of Shaanxi Province,Lintong 710600,China)

【机构】 陕西省科学院酶工程研究所陕西省酿造发酵产品质量监督检验站陕西省酶工程技术中心

【摘要】 以玉米粉-麸皮糖化液和葡萄糖-土豆汁为2种不同发酵主料,对益生菌产阮假丝酵母菌MYS-J1在70L全自动发酵罐条件下的发酵规律进行了深入研究,结果表明:在70L全自动发酵罐中装填系数为60%,温度30℃,接种量3%,通气量为1.5m3/h,罐压0.04MPa的条件下,酵母菌在玉米粉-麸皮糖化液下发酵时,最佳发酵水平可达6.46×108cfu/mL;酵母菌在葡萄糖-土豆汁中发酵时,最高发酵水平可达4.55×108cfu/mL。另外,总结了产阮假丝酵母菌MYS-J1在发酵罐中的生长曲线以及发酵过程中pH值、含糖量、生物量、固形物含量的变化趋势。

【Abstract】 The fermentation ofprobiotic Candidautilis MYS-J1 in 70 Lautomatic fermentor were investigated in cornmeal-bran syrup or glucose-murphy juice medium.It was shown that the highest fermentation level of C.utilis MYS-J1 was 6.46×108 cfu/ml and 4.55×108 cfu/ml in cornmeal-bran syrup medium and glucose-murphy juice medium,respectively,when the medium volume was 60% of fermentor,inoculum was 3%,and the fermentation was conducted at 30℃ with ventilation volume of 1.5 m3/h and fermentor presser of 0.04 MPa.In addition,the growth curve of C.utilis MYS-J1 and the variation of pH,sugar content,biomass and solid content during fermentation were also summarized.

【基金】 国家星火计划项目(2007EA850003);陕西省科学院产业化项目(2007K-12)
  • 【分类号】S816
  • 【被引频次】1
  • 【下载频次】177
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