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益生菌产阮假丝酵母菌MYS-J1的液体通风发酵研究
Aerobic submerged fermentation of probiotic Candida utilis MYS-J1
【摘要】 以玉米粉-麸皮糖化液和葡萄糖-土豆汁为2种不同发酵主料,对益生菌产阮假丝酵母菌MYS-J1在70L全自动发酵罐条件下的发酵规律进行了深入研究,结果表明:在70L全自动发酵罐中装填系数为60%,温度30℃,接种量3%,通气量为1.5m3/h,罐压0.04MPa的条件下,酵母菌在玉米粉-麸皮糖化液下发酵时,最佳发酵水平可达6.46×108cfu/mL;酵母菌在葡萄糖-土豆汁中发酵时,最高发酵水平可达4.55×108cfu/mL。另外,总结了产阮假丝酵母菌MYS-J1在发酵罐中的生长曲线以及发酵过程中pH值、含糖量、生物量、固形物含量的变化趋势。
【Abstract】 The fermentation ofprobiotic Candidautilis MYS-J1 in 70 Lautomatic fermentor were investigated in cornmeal-bran syrup or glucose-murphy juice medium.It was shown that the highest fermentation level of C.utilis MYS-J1 was 6.46×108 cfu/ml and 4.55×108 cfu/ml in cornmeal-bran syrup medium and glucose-murphy juice medium,respectively,when the medium volume was 60% of fermentor,inoculum was 3%,and the fermentation was conducted at 30℃ with ventilation volume of 1.5 m3/h and fermentor presser of 0.04 MPa.In addition,the growth curve of C.utilis MYS-J1 and the variation of pH,sugar content,biomass and solid content during fermentation were also summarized.
【Key words】 probiotics; Candida utilis; automatic fermentor; growth curve; fermentation property;
- 【文献出处】 中国酿造 ,China Brewing , 编辑部邮箱 ,2009年01期
- 【分类号】S816
- 【被引频次】1
- 【下载频次】177