【摘要】 目的:探讨人参二醇组皂苷(Panaxadiol saponins,PDS)对动脉粥样硬化大鼠血脂、血清NO、MDA含量、SOD活力的影响。方法:高脂饮食加维生素D3(总量7×10~5 U/Kg,分别于实验第1周和第6周腹腔注射),建立大鼠动脉粥样硬化模型。选用健康Wistar大鼠45只,体重180～230 g,雌雄不限。按清洁级动物分笼喂养,适应性喂养1周后随机分为3组:正常对照组,以普通饲料喂养;高脂模型组,以高脂饲料喂养;PDS给药组,高脂饲料喂养,同时每日1次PDS[100 mg/(kg·d)]灌胃,前两组以相应生理盐水灌胃,每周称重1次,并根据体重变化调整给药量。11周后颈动脉采血,检测血脂水平,血清一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果:1、PDS对血脂的影响:与对照组相比较,模型组大鼠血清TC、LDL-c及AI显著升高(P<0.01);与模型组比较,PDS给药组TC、LDL-c和AI明显降低,差异显著(分别P<0.01,P<0.01,P<0.05);而血清甘油三脂、高密度脂蛋白水平相比无统计学意义。2、PDS对血清NO含量的影响:与对照组相比较,模型组大鼠血清NO含量明显降低,P<0.01;与模型组比较,PDS组NO含量明显增高,P<0.05;药物组与对照组相比无统计学意义。3、PDS对血清MDA含量与SOD活力的影响:模型组MDA水平与对照组相比明显升高,P<0.01;而SOD活力明显降低,P<0.01。与模型组相比,PDS对血清SOD活力有明显升高的作用,P<0.05;同时,PDS具有显著降低MDA水平的作用,P<0.05。PDS组与对照组相比无统计学意义。结论:1、PDS通过降低大鼠血清总胆固醇、低密度脂蛋白水平,发挥其抗AS作用。2、PDS能够提升高脂大鼠血清NO水平,从而保护血管内皮,抑制AS的形成。3、PDS能够提高血清SOD活力,降低MDA含量,增强清除氧自由基和抗脂质过氧化功能,从而稳定细胞膜,保护内皮而发挥抗AS作用。更多还原

【Abstract】 Objective:To investigate the effects of Panaxadiol saponins on lipemia、NO 、MDA and SOD in the atherosclerosis Rats.Methods:Experimental atherosclerosis in rats was produced by feeding with high fat diet and administering vitamin D3 (7 × 10~5 U/Kg) in intraperitoneal injection at the first week and the sixth week respectively.Healthy forty-five male or female Wistar rats, whose body weights range from 180g to 230g,were randomized into 3 groups after being well adapted to a new life for a week.The three groups were normal group (feed on normal diet),AS model group (feed on high fat diet) and PDS group (feed on high fat diet and PDS 100mg/kg · d).The former two groups were given isotonic sodium chloride instead of PDS accordingly.The rats of each group were weighed once a week and the dosages were adjusted to the changes of body weight accordingly.After the rats were fed for successive 11 weeks,serum levels of total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C),Nitric oxide (NO),Superoxide dismutase (SOD),Malondialdehyde (MDA) were detected respectively.Results:1.The effect of PDS on blood lipoproteins:The serum level of TC 、LDL-c and AI of Model group was significantly higher than that of Control group(P <0.01);PDS decreased the serum level of TC,LDL-c,and AI significantly. The difference of each index between Model group and PDS group was remarkable (P < 0.01,P < 0.01,P < 0.05) but there was no sta- tistic difference between them in TG and HDL-c.2.The effect of PDS on the serum NO level:The serum NO level of PDS group was higher than that of model group (P < 0.05).The NO level of control group was higher than that of model group (P < 0.01);The difference in NO level between PDS group and control group was not remarkable statistically.3.The effects of PDS on the serum MDA level and SOD activity:MDA level of model group increased obviously compared with that of control group(P < 0.01).SOD activity of Model group decreased obviously compared with that of control group(P <0.01).PDS highly improved the serum SOD activity compared with model group (P < 0.05) and highly decreased the serum MDA level compared with model group (P < 0.05).The differences in MDA level and SOD activity between PDS and control group were not remarkable statistically.Conclusions:1.By decreasing serum levels of TC,LDL-c,PDS would resist AS.2.PDS would protect vascular endothelium to prevent atherosclerosis by improving serum levels of NO.3.PDS would resist lipid peroxidation to protect membrane by improving activity of SOD and decreasing the level of MDA.PDS would protect vascular endothelium and prevent AS in this way.更多还原