【摘要】 麦胚凝集素是麦胚中一种具有生物学活性蛋白质。根据甲壳素和麦胚凝集素的性质,以甲壳素作为吸附固相,建立了从麦胚中提取、分离和纯化麦胚凝集素的绢丝布袋法工艺。试验结果显示,采用40%~45%的饱和硫酸铵制备粗麦胚凝集素样品时,粗品麦胚凝集素溶液与布袋中甲壳素的使用比例在3∶1~1∶1,甲壳素吸附麦胚凝集素的时间为19 h,SDS-PAGE凝胶电泳显示可获得纯化度近100%的麦胚凝集素样品。纯化的麦胚凝集素相对分子质量为20415。更多还原

【Abstract】 Wheat germ agglutinin is a kind of protein with biological activity.Based on the characteristics of chitosan and wheat germ agglutinin,chitosan was saved as solid phase of absorption,and a spun silk bag method of extracting,separating and purifying agglutinin from wheat germ was developed.The purity of wheat germ agglutinin was identified with SDS gel electrophoresis.Results: Firstly the rough purification of wheat germ agglutinin is conducted with 40%～45% saturated ammonium sulfate,and the ratio of the roughly purified wheat germ agglutinin liquid and chitosan in the spun silk bag is at 3∶1～1∶1,the time of absorbing wheat germ agglutinin is 19 h.The isolated wheat germ agglutinin has a purity of about 100% with a relative molecule weight of 20415.更多还原