【摘要】 目的研究脊髓神经干细胞定向分化为神经元过程中细胞骨架蛋白——β-微管蛋白-Ⅲ(β-tubulin-Ⅲ)的表达及意义。方法采用细胞培养技术、免疫细胞化学(SABC)技术鉴定培养细胞,免疫荧光技术观察β-微管蛋白-Ⅲ在脊髓神经干细胞定向分化为神经元过程中的分布情况;采用Western blotting检测β-微管蛋白-Ⅲ在神经干细胞分化过程中的变化。结果分化第1天,β-微管蛋白-Ⅲ表达较弱;随着细胞的发育,分化第4天时β-微管蛋白-Ⅲ表达增强,其阳性反应物分布于核周,并伸出突起;β-微管蛋白-Ⅲ的含量也随着细胞的生长而增多;然而分化第7天时,β-微管蛋白-Ⅲ表达减少。结论在脊髓神经干细胞定向分化为神经元过程中,细胞骨架蛋白——β-微管蛋白-Ⅲ影响和促进细胞的分化。更多还原

【Abstract】 Objective To study about the expression and significance of β-Tubulin-Ⅲ in directed differentiation of neural cells from spinal neural stem cells.Methods Cell culture technology,immunocytochemistry(SABC) technique were used to identify the cultured cells.The distributions of β-Tubulin-Ⅲ by immunofluorescence were observed in the process of the spinal cord neural stem cell differentiation of neurons.In addition,we detected β-Tubulin-Ⅲ by Western blotting. Results There was weak expression of β-Tubulin-Ⅲ in the first day.During the growth of neuron,the expression of β-Tubulin-Ⅲ was increasing and distributed around the cell nucleus and into the process in the 4th day.The content of β-Tubulin-Ⅲ was also increasing with the growth of neurons.However,the expression of β-Tubulin-Ⅲ in the 7th day was lower than the 4th day.Conclusions During directed differentiation of spinal derived-neural stem cells to neurons,β-Tubulin-Ⅲ effect and promote the differentiation of neuron.更多还原