【摘要】 目的探讨骨髓间充质干细胞(MSCs)诱导后促进胰腺损伤修复的方法。方法应用贴壁法分离、纯化大鼠MSCs,经bFGF、EGF、HGF、2%B27、activeA、β巯基乙醇、尼克酰胺诱导12～14d,RT-PCR鉴定后,以DAPI标记治疗胰腺损伤模型大鼠。治疗1w后激光共聚焦显微镜观察胰岛样细胞的迁移、定位,RT-PCR鉴定参与胰腺损伤修复的细胞来源。结果MSCs经诱导12～14d,分化为可表达Ins1、Ins2的胰岛样细胞,经DAPI标记后治疗胰腺损伤模型大鼠1w,使炎性细胞浸润明显、细胞结构不清、胰岛结构崩解的损伤区的胰腺组织结构开始恢复,胰岛再建;RT-PCR检测Sry进一步证实损伤区胰腺组织内存在输注的胰岛样细胞。结论MSCs可诱导分化成胰岛样细胞,该细胞参与损伤胰腺组织的修复。更多还原

【Abstract】 Objective To treat pancreatic injury of model rats induced by bone marrow mesenchymal stem cells(BM-MSCs)in vitro,and explore methods promoting repair of pancreatic injury.MethodsBM-MSCs were segregated and purified by adherence method to in rats,then used to treat pancreatic injury of model rats after induced by basic fibroblast growth factor(bFGF),epidermal growth factor(EGF),hepatocyte growth factor(HGF),2%B27,active A,β-mercaptoethanol and nicotinamide 12[14 days,identified by RT-PCR,marked by DAPI.One week later,the migrations and locations of islet-like cells were observed by Laser Scanning Confocal Microscope(LSCM),and the source of cells participating in reparation of pancreatic injury was identified by RT-PCR.ResultsAfter 12～14 days,BM-MSCs were induced into the islet-like cells expressing Ins1 and Ins2.The islet-like cells were used to treat pancreatic injury after marked DAPI.One week later,the cells were discovered in injured pancreatic tissues,where inflammatory cells infiltration obviously,cell structure confusion,and pancreatic islet disintegration,and the injured pancreatic tissues began to recover,pancreatic islet re-established.The result of RT-PCR further confirmed that there were infused islet-like cells in injured pancreatic tissues.ConclusionsBM-MSCs can be induced into islet-like cells,which participate in reparation of pancreatic injury.更多还原