【摘要】 目的应用虫草菌丝对糖尿病肾病(DN)模型大鼠进行干预,探讨其作用效果及可能的作用机制。方法将Wistar大鼠分成正常组和糖尿病(DM)组,DM组大鼠给予链脲佐菌素(STZ)55mg/kg腹腔内注射,成模后随机分为模型组和治疗组每组大鼠分别在第4、8、16周各处死10只,在处死前留取24h尿量测24h尿白蛋白和尿肌酐,留取血标本离心后测血肌酐,留取肾脏标本用免疫组化和实时荧光定量PCR方法测胱抑素(CC),组织蛋白酶B(CB),Ⅳ型胶原(ColⅣ)和纤维连接蛋白(FN)的表达。结果与正常组比较模型和治疗组大鼠内生肌酐清除率(Ccr)和24h尿白蛋白第4周开始升高(P<0.01),免疫组化和PCR结果显示CB、CC、ColⅣ、FN在第8周出现明显上调(P<0.01或P<0.05),模型组CB在16w下调(P<0.01),与模型组相比,治疗组的CB,CC,ColⅣ,FN在前8周无显著变化,第16周CC,ColⅣ,FN表达下调(P<0.01或P<0.05),CB的表达上调(P<0.01)。结论虫草菌丝通过调节CB、CC的平衡来减少DN细胞外基质(ECM)的沉积,起到对DN的保护作用。更多还原

【Abstract】 Objective To study the effects of cordyceps sinensis on renal expressions of cathepsin B(CB)and cystatin C(CC)in diabetic nephropathy(DN)rats,and investigate its mechanisms.Methods100 healthy male Wistar rats were divided into normal and diabetic groups.The diabetic rat models were established by intraperitoneal injection of STZ(55 mg/kg),then the diabetic model rats were divided into model and treatment groups.At the end of 4,8 and 16 weeks,the urine of each group rats was collected,then 10 rats of each group were sacrificed and the kidneys and blood of them were collected.The expressions of CB,CC,ColⅣ and fibronectin(FN)in renal tissue were measured by real time PCR and immunohistochemical technique.ResultsCompared with normal group,the levels of Ccr and urine protein excretion of model group and treatment group were increased at 4 weeks(P<0.01),the expressions of CB,CC,ColⅣ and FN were significantly increased at 8 weeks(P<0.01,P<0.05),but the expression of CB in model group at 16 weeks was significantly decreased(P<0.01).Compared with model group,the expressions of CC,ColⅣ and FN in treatment group were significantly decreased at 16 weeks(P<0.01,P<0.05),but the expression of CB was significantly increased(P<0.01).ConclusionsCordyceps sinensis can reduce the excessive accumulation of ECM in the kidney of DN rats through balancing the expressions of CB and CC.更多还原