【摘要】 目的探讨通心络超微粉对大鼠心肌梗死后心室重塑(LVRM)及其病理和心肌线粒体超微结构的影响。方法130只SD大鼠随机取14只分为假手术组(0.9%NaCl灌胃,n=14,至实验终点时n=12)4w后处死。116只SD大鼠结扎冠状动脉前降支,术后存活的38只大鼠随机分为:急性心肌梗肆(AMI)模型组(0.9%NaCl灌胃,n=12)和通心络治疗组(1.0g.kg-1.d-1,n=13),卡托普利治疗组(15mg.kg-1.d-1,n=13)。心脏标本固定和病理分析,HE和Masson染色评价形态学特点。对术后2d和4w心脏超声数据分别进行分析。药物治疗4w后,用电子显微镜观察心肌细胞线粒体的变化。结果模型组和两治疗组间心肌梗死面积无明显差异,且三组大鼠术后2d和假手术组比较,左室舒张末期内径(LVEDD)明显增大(均P<0.001),而射血分数(EF)和短轴缩短率(FS)明显缩小(均P<0.001)。药物治疗4w后与模型组相比,两治疗组EF和FS明显增大(P<0.001),而左室舒张末期内径(LVEDD)明显缩小(P<0.001)。与术后2d相比,4w后模型组LVEDD明显增大(P<0.001),而两治疗组LVEDD明显减小(P<0.001)和EF和FS明显增加(P<0.001)。结论通心络超微粉可预防大鼠AMI后LVRM和改善心功能,这些作用基于通心络超微粉抗氧化应激作用。更多还原

【Abstract】 Objective To investigate the effects of Tongxinluo supermicropowder on left ventricular remodeling(LVRM) and the chances of myocardium pathology and mitochondria super-microcosmic structure in the rats with acute myocardial infarction(AMI). Methods 14 SD rats from 130 SD rats were randomly selected into sham operation group (fed with 0.9%NaCl, n=14, after 4 weeks, n=12), and were killed after 4 weeks. 116 SD rats underwent the left descending coronary artery ligation and 38 surviving rats were randomly assigned to AMI model group(fed with 0.9%NaCl, n=12),Tongxinluo supermicropowder treatment group (fed with Tongxinluo supermicropowder 1.0 g·kg-1·d-1, n=13) and captopril treatment group (fed with captopril 15 mg·kg-1·d-1, n=13). Morphological characteristics of rats′ myocardium were evaluated with HE, Masson stainning.The respective data of echocardiogram after 2 days and 4 weeks of surgery were analyzed. The changes of per-microcosmic structure of myocardial cell mitochondria were observed with electronic microscope after 4 weeks of treatment. Results There were no significant differences among model group and two treatment groups in infarction area. Compared with sham operation group after 2 days of surgery, the left ventricular end diastolic diameter (LVEDD) in model group and the two treatment groups were all significantly increased (all P<0.001), while the ejection fraction (EF) and shortening fraction (FS) were significantly decreased(all P<0.001). After 4 weeks of therapy, compared with model group, the EF and FS were significantly increased (P<0.001) while LVEDD were significantly decreased(P<0.001) in two treatment groups. Compared with after 2 days of surgery, LVEDD was significantly increased (P<0.001) in model group after 4 weeks of surgery, while LVED were significantly decreased (P<0.001) in two treatment group and the EF and FS were significantly increased (P<0.001) in two treatment groups. Conclusions Tongxinluo supermicropowder can prevent LVRM and improve cardiac function after AMI, which base on its antioxidation and anti-stress effects.更多还原