【摘要】 目的:观察survivin基因RNAi对宫颈癌裸鼠移植瘤生长与凋亡的影响。方法:随机将BALB/c裸鼠分4组,分别注射转染携survivin RNAi重组质粒的HeLa-s2细胞、转染阴性对照质粒细胞HeLa-NC、转染空质粒细胞HeLa-U6 neo及宫颈癌He-La细胞,建立人宫颈癌裸鼠皮下移植瘤模型。观察survivin RNAi对人宫颈癌裸鼠皮下移植瘤生长的影响;免疫组化法检测移植瘤组织中survivin蛋白的表达和微血管密度(MVD),H-E染色及TUNEL染色观察survivin RNAi对人宫颈癌裸鼠皮下移植瘤凋亡的影响。结果:成功建立人宫颈癌裸鼠皮下移植瘤模型,HeLa-s2组裸鼠瘤重明显小于其他3组(P<0.05);肿瘤生长抑制率为67.9%。免疫组化结果显示,HeLa-s2组裸鼠移植瘤组织中survivin蛋白表达显著下降;MVD值也显著降低(P<0.05);H-E染色、TUNEL染色结果显示,HeLa-s2组裸鼠移植瘤组织细胞凋亡明显增多(P<0.05),AI值达(22.73±1.37)%。结论:Sur-vivin基因RNAi可通过下调移植瘤组织survivin蛋白表达和降低其MVD抑制移植瘤生长并促进其凋亡。更多还原

【Abstract】 Objective: To observe the effect of RNAi targeting survivin gene(RNAi-survivin) on the growth and apoptosis of implanted human cervical carcinoma in mice.Methods: Nude mice were randomly divided into 4 groups,and the human cervical carcinoma HeLa cells transfected with pSilencer2.1-s2,pSilencer2.1-NC,pSilencer2.1-U6 neo or not transfected(the cells being HeLa-s2,HeLa-NC,HeLa-U6 neo and Hela,respectively) were subcutaneously inoculated into flank tissues of mice to establish cervical carcinoma mouse models.The influence of survivin-RNAi on the implanted tumor growth was observed.Expression of survivin protein and microvessel density(MVD) in implanted cervical carcinoma tissues were examined by immunohistochemistry.Apoptosis of implanted cervical carcinoma cells was observed by H-E staining and TUNEL.Results: Implanted human cervical carcinoma mouse model was successively established.The tumor weight in HeLa-s2 group was markedly lower than those in HeLa-NC,HeLa-U6 neo and Hela groups(P<0.05).The tumor inhibitory rate in HeLa-s2 group was 67.9%.Survivin expression and MVD value in tumor tissues of HeLa-s2 group were significantly decreased as determined by immunohistochemistry(all P<0.05).Apoptosis of implanted cervical carcinoma cells in HeLa-s2 group was significantly increased compared with those in other 3 groups(all P<0.05),with AI being(22.73±1.37)%.Conclusion: RNAi-survivin inhibits the growth and promote apoptosis of implanted human cervical carcinoma tumors by down-regulating survivin protein expression and MVD in tumor tissues.更多还原