【摘要】 目的检测Twist、IGF-1蛋白在大肠癌中的表达,分析与临床病理学因素的关系,探讨两者在大肠癌发病机制中的相互作用以及与患者预后的关系。方法选取不同类型的大肠癌组织48例,癌旁5cm以上的正常组织24例,采用SP法检测Twist、SABC法检测IGF-1的表达。结果在大肠癌组织中Twist、IGF-1的阳性表达率分别为47.92%、64.58%,均显著高于癌旁正常组织中的12.50%、16.67%。Twist、IGF-1在大肠癌组织中的表达与肿瘤的浸润程度、淋巴结转移、Dukes分期显著相关,Twist的表达还与患者5年生存情况有显著的相关性。Twist和IGF-1在大肠癌组织中的表达呈明显相关性。结论Twist、IGF-1可能协同作用参与大肠癌的浸润、转移,同时Twist阳性表达提示预后不良,对判断大肠癌患者的预后有一定的参考价值。更多还原

【Abstract】 Objective To investigate the expression of Twist and IGF-1 in colorectal carcinomas and the relation with clinical and pathological factors,and to analyze the interaction of Twist and IGF-1 on nosogenesis of colorectal carcinomas and the prognosis of patients.Methods Immunohistochemical SP methods was adopted to examine the expression of Twist and Immunohistochemical SABC methods was adopted to examine the expression of IGF-1 in colorectal carcinomas(48 cases) and paracancerous normal tissues(24 cases).Results The positive rates of Twist and IGF-1 in colorectal carcinomas(47.92% and 64.58%) were significantly higher than those in paracancerous normal tissues(12.50% and 16.67%).The overexpression of Twist and IGF-1 were significantly related with the tumor differentiation,the depth of invasion,lymph node metastasis and the Dukes stage.The overexpression of Twist was aslo significantly related with the 5 year survival state.The expression of Twist in colorectal carcinomas was obviously related with IGF-1.Conclusion The expression of Twist and IGF-1 may play a cooperative role in the infiltration and metastasis of colorectal carcinomas.The positive expression of Twist indicates poor prognosis and provide a valuable reference for the decision of prognosis.更多还原