【摘要】 目的探讨alpha B-crystallin基因在结肠癌组织中的表达及其与结肠癌临床病理的相关性。方法采用RT-PCR方法检测64例结肠癌组织及其癌旁正常组织中alpha B-crystallin基因的表达,计算其阳性率;并用Western blot方法检测结肠癌组织及其癌旁正常组织中alpha B-crystallin蛋白的表达变化。结果alpha B-crystallin mRNA在结肠癌组织中表达的阳性率明显高于癌旁正常组织(P<0.01),在发生浆膜外转移者的表达阳性率明显高于浆膜内者(P<0.05),在较大肿瘤者中的表达阳性率明显高于较小肿瘤者,在有淋巴结转移者中的表达阳性率明显高于无淋巴结转移者(P<0.05),但与患者的性别、年龄、肿瘤的分化程度无关(P>0.05)。alpha B-crystallin蛋白在结肠癌组织中表达明显高于癌旁正常组织(P<0.01)。结论alpha B-crystallin基因的高表达可能与结肠癌的发生及侵袭转移有关。更多还原

【Abstract】 Objective To study the expression of alpha B-crystallin in colon carcinoma,and its relation in clinical pathology of colon carcinoma.Methods The expression of alpha B-crystallin mRNA was detected using RT-PCR in 64 colon carcinoma tissues and adjacent normal tissues.The expression level of alpha B-crystallin protein was detected by RT-PCR and Western blot.The detection rates of alpha B-crystallin mRNA and protein expression in cancer tissues and adjacent normal tissues were analyzed.Results Alpha B-crystallin mRNA expression rate in cancer tissues was significantly higher than adjacent normal tissues(P<0.01).The patients with colon cancer involving the serosa obtained obviously higher alpha B-crystallin expression rate than the one not involving the serosa,The group which has bigger tumor or with lymph node metastasis has higher expression of alpha B-crystallin than those group with smaller size of tumor or without lymph node metastasis(P<0.05),and its expression has no correlation with age,sex and tumor differentiation(P>0.05).The protein of alpha B-crystallin was detected in cancer tissues but not in adjacent normal tissues(P<0.01) by Western blotting.Conclusion The expression of alpha B-crystallin is positively correlated with tumorogenesis and progression of colon cancer.It may be used as a good marker for clinic therapeutic purpose and prognosis estimation of colon cancer.更多还原