【摘要】 目的探讨胃癌组织胸苷酸合成酶增强子区(TSER)多态性与5-Fu体外药敏的关系。方法对45例胃癌患者新鲜标本进行体外肿瘤细胞原代培养,用ATP-TCA法检测肿瘤细胞对5-Fu的敏感性并用PCR法检测肿瘤的TSER多态性,分析TSER多态性分布频率与体外药敏有效率的关系。结果TSER多态性三种基因型频率分布:2R/2R、2R/3R、3R/3R基因组分别为6.7%(3/45)、31.1%(14/45)和62.2%(28/45)。5-Fu总有效率为33.3%(14/42)。3R/3R基因组26例,有效5例,无效21例,有效率19.2%;(2R/2R、2R/3R)基因组16例,有效9例,无效7例,有效率56.3%。TSER基因型组之间对5-Fu敏感性存在显著差异性(P<0.05)。结论TSER多态性对5-Fu化疗敏感性可能有关,基因型检测可能有助于预测胃癌组织对5-Fu化疗敏感性。更多还原

【Abstract】 Objective To investigate correlation between thymidylate synthase enhancer region(TSER) polymorphism and the antitumor activities of 5-fluorouracil(5-Fu) determined by ATP sensitivity assay in vitro.Methods The carcinoma cells isolated from fresh gastric tissue in 45 patients were cultured in vitro.The sensitivity of these cells to 5-Fu were tested using ATP-TCA method.TSER genotypes were detected by PCR and the correlation between different of TSER genotypes and its chemosensitivity to 5-Fu was analyzed in this study.Results In all cases,the frequency distribution of TSER 2R/2R、2R/3R and 3R/3R genotypes were 6.7%(3/45)、31.1%(14/45) and 62.2%(28/45),respectively.The overall effective rate of 3R/3R group to 5-Fu was 33.3%(14/42).In 2R/2R and 2R/3R genotype group its effective rate to 5-Fu was 56.3%(9/16),which was significantly higher than that of 3R/3R genotype group(19.2%,P<0.05).Conclusions The results in the present study suggest that the polymorphism of TSER was associated with the antitumor activities of 5-fluorouracil,indicating that analysis of TSER polymorphisms might be useful to direct 5-fluorourcil-based antitumor chemotherapy.更多还原