【摘要】 考察了不同温度(29~34℃)对红色糖多孢菌(Saccharopolyspora erythraea)合成红霉素(erythromycin)的影响,对不同温度下的发酵过程进行动力学特性分析,在此基础上提出了红霉素合成的变温培养方法:延滞期及对数期初期温度控制在33℃,发酵中期32℃,后期降温至29℃的条件下进行培养。采用此变温培养进行发酵,红霉素的化学效价和生物效价比恒温32℃培养对照组分别提高了24%和11.1%。更多还原

【Abstract】 Fermentation for Erythromycin production by Saccharopolyspora erythraea HA at various temperature between 29℃ and 34℃ was investigated and the dynamics characteristics during fermentation process were also analyzed.Based on the results,a varying temperature nursing method of three stage control strategy is proposed: During the first stage,which comprises the lag phase and the index phase,the temperature is maintained at 33℃;then the temperature is shifted to 32℃ in the ferment middle phase and dropped to 29℃ at the end of the fermentation process.By this method,the erythromycin titres of biological and are chemical assays 11.1% and 24% higher than that of fixed temperature(32℃) fermentation.更多还原