【摘要】 目的观察诱导分化为心肌样细胞的人骨髓间充质干细胞(hMSCs)是否可产生动作电位。方法体外分离培养hM-SCs,5-氮胞苷诱导向心肌细胞(CMs)分化。分别以体外分离培养的原代搏动的CMs和同代次的hMSCs为阳性对照和阴性对照,利用膜片钳技术观察动作电位。结果在30个诱导分化成心肌样细胞的hMSCs中有6个细胞可诱发出动作电位;CMs具有典型的动作电位;未经诱导的hMSCs没有引出动作电位,5-氮胞苷诱导后细胞可以引出微弱的动作电位。结论实验结果表明hMSCs经5-氮胞苷诱导后,在电学特性方面具有向CMs分化的潜能;与CMs具有的电生理特性相似,这些心肌样细胞具有兴奋性。更多还原

【Abstract】 Objective To establish the model of glioma by hypodermic of C6 cells line into the nude mice and observe the growth characteristics.Methods The C6 gliocyte suspension was injected into the left subaxillary hypoderma of nude mice.The diameter and the volume of the tumor were measured and calculated,and the volume-time growth curve was plotted.The cells of tumor were observed under the HE and glial fibrillary acidic protein (GFAP) immono-histochemistry staining.Results The tumor appeared in inoculation area 7 d after injection,and presented nearly exponential development after about 20 d.The survival time of the nude mice is 22~48 d.The tumors were with sharp border,affluent blood vessel,and a great number of GFAP-positive cells.Conclusion The model of glioma can be well induced by hypodermic of the cultured C6 gliocyte into nude mouse.更多还原