【摘要】 目的初步探讨丝裂原激活的蛋白激酶(MAPKs)3条主要信号通路在大鼠脑缺血预适应中(IPC)的作用。方法将大鼠随机分为正常对照(control)、假手术(SH)、缺血预适应/缺血耐受(IT)3组,每组6只。应用SDS-PAGE、Westernblot等实验方法,借助GelDoc和VersaDoc凝胶成像系统,半定量检测大鼠大脑躯体感觉皮层ERK、JNK、p38磷酸化水平和蛋白表达量的变化。结果IT组大鼠躯体感觉皮层内ERK1和JNK46KD的磷酸化水平增高(P<0.05)。结论大鼠躯体感觉皮层内MAPKs信号通路中ERK1和JNK46KD激活可能参与了脑缺血预适应的形成,但也不能排除p38在其中的作用。更多还原

【Abstract】 Objective To explore initially the roles of the 3 major signaling pathways of mitogen activated protein kinases(MAPK)in cerebral ischemia preconditioning.MethodsHealthy adult SD rats were randomly divided into 3 groups:normal control group;sham-operated group and ischemic preconditioning or ischemic tolerance group(n=6).SDS-PAGE,Western blot and Gel Doc imagine systems were applied to determine the phosphorylation and protein expression of ERK,JNK and p38 in somatosensory cortex of rat.ResultsThe phosphorylation level of ERK1 and JNK46KD in somatosensory cortex increased significantly(P<0.05)after ischemia preconditioning.ConclusionThe increased ERK1 and JNK46KD phosphorylation in somatosensory cortex may be involved in the development of cerebral ischemia preconditioning and can not be ruled out in which the role of p38.更多还原