【摘要】 CD25是CD4+CD25+调节性T细胞的重要标记分子.树鼩是一种重要的人类疾病动物模型,但是,树鼩的CD25基因还是未知.我们以树鼩脾脏RNA为模板,对树鼩白介素-2受体基因alpha链(tsCD25)进行了RT-PCR(逆转录-聚合酶链式反应),获得了一个816bp的开放阅读框.TsCD25 cDNA编码一个由271个氨基酸组成的分子量为30.9 ku的蛋白多肽.预测的tsCD25包含2个Sushi结构域、2个N-糖基化位点和多个O-糖基化位点.tsCD25和灵长类CD25在氨基酸水平上的同源性为65.3%~66.4%.RT-PCR检测发现,tsCD25 mRNA分布于树鼩的外周血、脾脏和肺,并受PMA和ionomycin刺激的调节.我们的结果为下一步制备tsCD25单克隆抗体,抑制CD4+CD25+Tregs功能以及开展有关的研究工作奠定了基础.更多还原

【Abstract】 CD25 is an important cell surface marker for CD4+CD25+ regulatory T cells(Tregs) and tree shrew is an animal model used in the study of human viral infections.However,the molecular identity of tree shrew CD25(tsCD25) remains unclear.We cloned tsCD25 and analyzed its expression pattern.A full-length cDNA of tree shrew interleukin 2 receptor alpha chain(tsCD25) gene was obtained from tree shrew spleen by RT-PCR(reverse transcription-polymerase chain reaction).The tsCD25 cDNA contains an ORF of 816 bp,encoding a 30.9 ku of peptide with a predicted 271 amino acids.The mature tsCD25 peptide contains two Sushi domains,two N-linked and multiple O-linked glycosylation sites.The tsCD25 is very similar to those of primates at the amino acid level,ranging from 65.3% to 66.4%.Tissue transcription analysis indicates that tsCD25 mRNA is expressed in peripheral blood,spleen and lung.Lymphocytes expression analysis demonstrates that tsCD25 mRNA is up-regulated by PMA and ionomycin stimulation.Our results may provide a basis for future studies such as generating monoclonal antibodies to tsCD25.更多还原