【摘要】 目的:探讨趋化因子受体CX3CR1在系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中的表达,及其与疾病活动的相关性。方法:收集93例确诊的SLE患者和30例正常人的PBMC,提取RNA。应用RT-PCR方法检测CX3CR1mRNA表达水平,并与疾病活动度评分(SLEDAI)作直线相关性分析。结果:①CX3CR1 mRNA在PBMC中的表达水平,活动期(3.735±0.557)与非活动期(0.530±0.045),两者差异有统计学意义(t=2.606,P=0.01);非活动期与对照组(0.146±0.02)间的差异有统计学意义(t=5.831,P=0.000);患者组(2.819±1.347)与对照组间的差异有统计学意义(t=3.921,P=0.000)。②SLE患者组CX3CR1 mRNA水平与疾病活动度评分(SLEDAI)呈正相关(r=0.445,t=4.523,P<0.001)。③不具有血管炎、肌炎、肾损及血小板减少的患者与具有上述相应临床特征的患者比较,CX3CR mRNA表达水平更高,差异具有统计学意义。结论:CX3CR1mRNA表达水平在活动期SLE比非活动期增高,与疾病的活动性(SLEDAI)呈正相关。CX3CR1可能反映疾病的活动性、参与特异器官受累的发生。更多还原

【Abstract】 Objective: To assess the expressions of chemokine receptors CX3CR mRNA on peripheral blood mononuclear cell(PBMC) with active or inactive systemic lupus erythematosus(SLE).Methods: Ninty three definitive SLE patients and 30 controls were collected.PBMC were separated from the peripheral blood specimen.Reverse transcription-polymerase chain reaction(RT-PCR) technique was applied to the semi-quantitatively analyze for the expression of chemokine receptors mRNA(CX3CR1) in PBMC. Results: Level of CX3CR1 mRNA expression was significantly increased in patients with SLE(2.819±1.347)as compared with the controls(0.146±0.02)(t=3.921,P=0.000).Level of CX3CR1 mRNA expression in PBMC showed significantly positive correlation with disease activity(SLEDAI). Conclusion: CX3CR1 might be involved in the pathogenesis of SLE,and the expression level might be a good indicator for the disease activity of SLE.更多还原