【摘要】 目的:检测过敏性疾病患者血清Th1细胞因子IFN-γ和Th2细胞因子IL-4、IL-5、IL-13以及趋化因子Eotaxin、RANTES、LTB4的水平,探讨其临床意义。方法:过敏性疾病患者64例,正常对照21例。采用双抗体夹心酶联免疫吸附实验(ELISA)检测血清IL-4、IL-5、IL-13、IFN-γ、LTB4、RANTES和Eotaxin水平。结果:1过敏性疾病患者血清IL-4、IL-5、IL-13和Eotaxin、LTB4水平较对照组明显增高,差异有统计学意义(P<0.05)。2IFN-γ和RANTES在过敏性疾病患者血清中的水平低于对照组,但无统计学差异(P>0.05)。3IL-4、IL-5、IL-13和LTB4彼此之间呈显著正相关(P<0.01)。4Eotaxin、RANTES和IFN-γ彼此之间呈显著正相关(P<0.05)。5LTB4和Eotaxin之间呈显著正相关(P<0.01)。结论:过敏性疾病的发生存在多种细胞因子和趋化因子复杂的相互作用。更多还原

【Abstract】 Objective: To determine Th1/Th2 cytokines and chemokines in patients with allergic diseases and its clinic significance.Methods: Serum levels of IFN-γ,IL-4,IL-5,IL-13,Eotaxin,RANTES and LTB4 were determined from peripheral blood of 64 allergic patients and 21 healthy controls with ELISA.Results: IL-4,IL-5,IL13 and Eotaxin,LTB4 were increased significantly in serum of allergic patients compared with those of controls(P<0.05).There were no significant differences in serum levels of IFN-γ and RANTES between patients and controls(P>0.05).Serum levels of IL-4,IL-5,IL-13 and LTB4 were correlated with each other(P<0.01).Eotaxin,RANTES and IFN-γ levels were also significantly correlated with each other(P<0.05).LTB4 was correlated with Eotaxin as well(P<0.01).Conclusion: A wide range of cytokines and chemokines is involved in allergic diseases,which may play their roles in a complex interactive manner.更多还原