【摘要】 目的研究地塞米松对过氧化氢(H2O2)体外杀伤烟曲霉(Aspergillus fumigatus,A.fumigatus)的影响。方法用不同浓度的地塞米松(0 mg/mL,0.02 mg/mL,0.2 mg/mL)处理烟曲霉孢子,按照处理时间不同分为A(0.5 h),B(2 h),C(7 h),D(16 h)4组。用测定H2O2杀伤真菌的标准方法——斑点法分别测定各组烟曲霉孢子对H2O2的氧化杀伤敏感性。结果在H2O2浓度为1.5 mmol/L下,未用地塞米松处理的孢子(阴性对照)氧化杀伤敏感性为5×101(生长良好)。A(0.5 h)组:地塞米松0.02 mg/mL处理后孢子的氧化杀伤敏感性为5×103;地塞米松0.2 mg/mL处理后孢子的氧化杀伤敏感性为5×104。B(2 h)组:地塞米松0.02 mg/mL和0.2 mg/mL处理后孢子的氧化杀伤敏感性均为5×103。C(7 h)和D(16 h)组:不同浓度地塞米松处理后氧化杀伤敏感性与阴性对照没有差别(均为5×101)。结论地塞米松使H2O2在体外杀伤烟曲霉的能力增强,这种作用仅发生在地塞米松接触烟曲霉孢子的早期(<2 h)。更多还原

【Abstract】 Objective To study the effects of dexamethasone on hydrogen peroxide(H2O2) oxidative killing of Aspergillus fumigatus(A.fumigatus) in vitro.Methods A.fumigatus spores were administered with different concentrations(0 mg/mL,0.02 mg/mL,0.2 mg/mL) of dexamethasone and were divided into four groups(A:0.5 h,B:2 h,C:7 h,D:16 h) according to the treatment time.The H2O2 oxidative killing assay was performed,using spot test in the four groups.Results With 1.5 mmol/L of H2O2,the oxidative killing susceptibility of A.fumigatus without dexamethasone(negative control) was 5×101(grow well).Group A: the oxidative killing susceptibility of A.fumigatus treated with 0.02 mg/mL of dexamethasone was 5×103;and that with 0.2 mg/mL of dexamethasone was 5×104.Group B: the oxidative killing susceptibility of A.fumigatus treated with 0.02 mg/mL and 0.2 mg/mL of dexamethasone was all 5×103.Group C and Group D: the oxidative killing susceptibility of A.fumigatus with different concentration of dexamethasone had no difference with that of negative control(5×101).Conclusions Dexamethasone strenthens the ability of H2O2 to kill A.fumigatus in vitro,which happens only at the early stage(<2 h) of contacting with A.fumigatus spores.更多还原